Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit HELLS Polyclonal Antibody | anti-HELLS antibody
HELLS Antibody
Gene Names
HELLS; LSH; ICF4; PASG; SMARCA6; Nbla10143
Reactivity
Human, Mouse, Rat, MonkeyPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
HELLS; Polyclonal Antibody; HELLS Antibody; FLJ10339; Helicase lymphoid specific; Hells; HELLS_HUMAN; LSH; Lymphoid specific helicase; Lymphoid-specific helicase; Nbla10143; PASG; Proliferation associated SNF2 like protein; Proliferation-associated SNF2-like protein; SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; anti-HELLS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
HELLS Antibody detects endogenous levels of HELLS.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
838
Applicable Applications for anti-HELLS antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human HELLS.
Tissue Specificity
Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using HELLS Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: COS-7; Lane 4: HepG2; Lane 5: MCF7.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using HELLS Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: COS-7; Lane 4: HepG2; Lane 5: MCF7.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using HELLS Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using HELLS Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-HELLS antibody
Description: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity).
Similarity: Belongs to the SNF2/RAD54 helicase family.
Similarity: Belongs to the SNF2/RAD54 helicase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 97 kDa
Predicted: 98 kDa
Predicted: 98 kDa
NCBI Official Full Name
lymphoid-specific helicase isoform 1
NCBI Official Synonym Full Names
helicase, lymphoid specific
NCBI Official Symbol
HELLS
NCBI Official Synonym Symbols
LSH; ICF4; PASG; SMARCA6; Nbla10143
NCBI Protein Information
lymphoid-specific helicase
UniProt Protein Name
Lymphoid-specific helicase
Protein Family
UniProt Gene Name
HELLS
UniProt Synonym Gene Names
PASG; SMARCA6
UniProt Entry Name
HELLS_HUMAN
NCBI Description
This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]
Research Articles on HELLS
Similar Products
Product Notes
The HELLS hells (Catalog #AAA9611383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HELLS Antibody reacts with Human, Mouse, Rat, Monkey Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's HELLS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HELLS hells for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HELLS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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