Rabbit anti-Human IKB epsilon Polyclonal Antibody | anti-IKBepsilon antibody

IKB epsilon Polyclonal Antibody

Cat. Num. AAA3012875

• Gene Names: NFKBIE; IKBE
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: IKB epsilon; Polyclonal Antibody; IKB epsilon Polyclonal Antibody; NF-kappa-BIE; IkB-E; IkB-epsilon; IkappaBepsilon; I kappa B epsilon; I-kappa-B-epsilon; IkB E; IKBE; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35; member B2; anti-IKBepsilon antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IKB epsilon polyclonal antibody detects endogenous levels of IKB epsilon protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 500

• Applicable Applications for anti-IKBepsilon antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50-1:200
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of IKB epsilon on different cell lysate using anti-IKB epsilon antibody at 1/1,000 dilution. Positive control: Line 1: THP-1 Line 2: Jurkat Line 3: Hela)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. Counter stained with hematoxylin.)

Related Product Information for anti-IKBepsilon antibody

On the basis of both functional and structural considerations, members of the IkappaB family of proteins can be divided into four groups. The first of these groups, IkappaB-alpha, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kappaB sites, suggesting that the IkappaB-alpha family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkappaB family is represented by a protein designated IkappaB-beta. The third group of IkappaB proteins is represented by IkappaB-gamma, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IkappaB family member, IkappaB-epsilon, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.

NCBI and Uniprot Product Information

• NCBI GI #: 71274109
• NCBI GeneID: 4794
• NCBI Accession #: NP_004547.2
• NCBI GenBank Nucleotide #: NP_004547.2
• UniProt Accession #: O00221
• Molecular Weight: 45kDa
• NCBI Official Full Name: NF-kappa-B inhibitor epsilon
• NCBI Official Synonym Full Names: NFKB inhibitor epsilon
• NCBI Official Symbol: NFKBIE
• NCBI Official Synonym Symbols: IKBE
• NCBI Protein Information: NF-kappa-B inhibitor epsilon
• UniProt Protein Name: NF-kappa-B inhibitor epsilon
• UniProt Gene Name: NFKBIE
• UniProt Synonym Gene Names: IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon
• UniProt Entry Name: IKBE_HUMAN

NCBI Description

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor; D-serine transport