Rabbit ALDH5A1 Polyclonal Antibody | anti-ALDH5A1 antibody

Rabbit Polyclonal to Human ALDH5A1

Cat. Num. AAA244135

• Gene Names: ALDH5A1; SSDH; SSADH
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ALDH5A1; Polyclonal Antibody; Rabbit Polyclonal to Human ALDH5A1; Anti-ALDH5A1 Antibody (C-Terminus) IHC-plus; SSADH; SSDH; Human ALDH5A1; anti-ALDH5A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 535

• Applicable Applications for anti-ALDH5A1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: Aldh5A1 antibody can be used for detection of Aldh5A1 by Western blot at 1-2 ug/ml
• Target Species: Human
• Immunogen Description: A 22 amino acid peptide near the carboxy terminus of human Aldh5A1.
• Immunogen Type: Synthetic peptide
• Immunogen: ALDH5A1 antibody was raised against a 22 amino acid peptide near the carboxy terminus of human Aldh5A1.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-ALDH5A1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ALDH5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of Aldh5A1 in mouse liver lysate with Aldh5A1 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507229
• NCBI GeneID: 7915
• NCBI Accession #: NP_001071.1
• NCBI GenBank Nucleotide #: NM_001080.3
• UniProt Accession #: P51649; Q546H9; Q8N3W6; B2RD26; G5E949
• Molecular Weight: 58,653 Da
• NCBI Official Full Name: succinate-semialdehyde dehydrogenase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 5 family, member A1
• NCBI Official Symbol: ALDH5A1
• NCBI Official Synonym Symbols: SSDH; SSADH
• NCBI Protein Information: succinate-semialdehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase 5 family member A1; mitochondrial succinate semialdehyde dehydrogenase; NAD(+)-dependent succinic semialdehyde dehydrogenase
• UniProt Protein Name: Succinate-semialdehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH5A1
• UniProt Synonym Gene Names: SSADH
• UniProt Entry Name: SSDH_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ALDH5A1: Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency). SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. Belongs to the aldehyde dehydrogenase family.

Protein type: EC 1.2.1.24; Mitochondrial; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Oxidoreductase

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; succinate-semialdehyde dehydrogenase activity; succinate-semialdehyde dehydrogenase [NAD(P)+] activity

Biological Process: succinate metabolic process; glutamate metabolic process; central nervous system development; neurotransmitter catabolic process; short-chain fatty acid metabolic process; neurotransmitter secretion; acetate metabolic process; glucose metabolic process; glutamine metabolic process; glucosylceramide metabolic process; protein homotetramerization; post-embryonic development; synaptic transmission; glutathione metabolic process; galactosylceramide metabolic process; glycerophospholipid metabolic process; gamma-aminobutyric acid catabolic process

Disease: Succinic Semialdehyde Dehydrogenase Deficiency