Western Blot (WB)
(Western Blot analysis of SNF8 expression in transfected 293T cell line by SNF8 polyclonal antibody. Lane 1: SNF8 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human SNF8 Polyclonal Antibody | anti-SNF8 antibody
SNF8 (Vacuolar-sorting Protein SNF8, ELL-associated Protein of 30kD, EAP30, ESCRT-II Complex Subunit VPS22, hVps22, Dot3) (PE)
Gene Names
SNF8; Dot3; EAP30; VPS22
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SNF8; Polyclonal Antibody; SNF8 (Vacuolar-sorting Protein SNF8; ELL-associated Protein of 30kD; EAP30; ESCRT-II Complex Subunit VPS22; hVps22; Dot3) (PE); anti-SNF8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SNF8.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
1226
Applicable Applications for anti-SNF8 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human NF8, aa1-258 (NP_009172.2).
Immunogen Sequence
MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SNF8 expression in transfected 293T cell line by SNF8 polyclonal antibody. Lane 1: SNF8 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of SNF8 expression in transfected 293T cell line by SNF8 polyclonal antibody. Lane 1: SNF8 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-SNF8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens SNF8, ESCRT-II complex subunit (SNF8), mRNA
NCBI Official Synonym Full Names
SNF8 subunit of ESCRT-II
NCBI Official Symbol
SNF8
NCBI Official Synonym Symbols
Dot3; EAP30; VPS22
NCBI Protein Information
vacuolar-sorting protein SNF8
Protein Family
NCBI Description
The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Research Articles on SNF8
Similar Products
Product Notes
The SNF8 (Catalog #AAA6394618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNF8 (Vacuolar-sorting Protein SNF8, ELL-associated Protein of 30kD, EAP30, ESCRT-II Complex Subunit VPS22, hVps22, Dot3) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SNF8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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