Rabbit anti-Human, Mouse IGFBP-3 Polyclonal Antibody | anti-IGFBP-3 antibody

IGFBP-3 (Phospho-Ser183) Antibody

Cat. Num. AAA9502831

• Gene Names: IGFBP3; IBP3; BP-53
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: IGFBP-3; Polyclonal Antibody; IGFBP-3 (Phospho-Ser183) Antibody; IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor; anti-IGFBP-3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: IGFBP-3 (Phospho-Ser183) Antibody detects endogenous levels of IGFBP-3 only when phosphorylated at Ser183.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-IGFBP-3 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S183; M:S183
• Immunogen: The antiserum was produced against synthesized peptide derived from human IGFBP-3 around the phosphorylation site of Ser183.
• Immunogen Range: 151-200
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using IGFBP-3 (Phospho-Ser183) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 62243068
• NCBI GeneID: 3486
• NCBI Accession #: NP_000589.2
• NCBI GenBank Nucleotide #: NM_000598.4
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 31 kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform b
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.