Rabbit IGF1 Polyclonal Antibody | anti-IGF1R antibody

IGF1 Receptor (IGF1R) Antibody (C-term)

Cat. Num. AAA9216899

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: IGF1; Polyclonal Antibody; IGF1 Receptor (IGF1R) Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD221; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; IGF1R; anti-IGF1R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Sequence Length: 1367

• Applicable Applications for anti-IGF1R antibody: Western Blot (WB), IImmunohistochemistry (IHC) Paraffin
• Application Notes: WB~~1:2000; IHC-P~~1:50~100
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-IGF1 Receptor (IGF1R) Antibody (C-term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: C2C12 whole cell lysateLane 4: C6 whole cell lysateLane 5: Hela whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 155 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-IGF1 Receptor (IGF1R) Antibody (C-term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 155 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of anti-IGF1R Pab in SK-BR-3 cell lysate. IGF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-IGF1R antibody

The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.

Product Categories/Family for anti-IGF1R antibody

Cancer; Developmental biology; Neuroscience; Signal transduction

References

Song, R.X., et al., Proc. Natl. Acad. Sci. U.S.A. 101(7):2076-2081 (2004). Zhao, H., et al., Oncogene 23(3):786-794 (2004). Lu, Y., et al., Biochem. Biophys. Res. Commun. 313(3):709-715 (2004). Hakam, A., et al., Dig. Dis. Sci. 48(10):1972-1978 (2003). Li, Y., et al., Arterioscler. Thromb. Vasc. Biol. 23(12):2178-2184 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.4
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor isoform 1
• NCBI Official Synonym Full Names: insulin like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13
• NCBI Protein Information: insulin-like growth factor 1 receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; receptor complex

Molecular Function: ATP binding; identical protein binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor receptor activity; phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity

Biological Process: brain development; epidermis development; exocrine pancreas development; immune response; inactivation of MAPKK activity; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; male sex determination; mammary gland development; negative regulation of apoptosis; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; protein tetramerization; regulation of JNK cascade; signal transduction

Disease: Insulin-like Growth Factor I, Resistance To