Rabbit Fibulin 3 Polyclonal Antibody | anti-EFEMP1 antibody

Fibulin 3 Antibody

Cat. Num. AAA150666

• Gene Names: EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Fibulin 3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Fibulin 3; Polyclonal Antibody; Fibulin 3 Antibody; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; anti-EFEMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
• Purity/Purification: Fibulin 3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 355

• Applicable Applications for anti-EFEMP1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.
• Buffer: Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Fibulin 3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.)

Related Product Information for anti-EFEMP1 antibody

Fibulin 3 Antibody: Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.

NCBI and Uniprot Product Information

• NCBI GI #: 9665262
• NCBI GeneID: 2202
• NCBI Accession #: NP_001034437
• NCBI GenBank Nucleotide #: NM_004105.3
• UniProt Accession #: Q12805; Q541U7; A8K3I4; B4DW75; D6W5D2
• Molecular Weight: Predicted: 52 kDa; ; Observed: 47 kDa
• NCBI Official Synonym Full Names: EGF containing fibulin-like extracellular matrix protein 1
• NCBI Official Symbol: EFEMP1
• NCBI Official Synonym Symbols: DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
• NCBI Protein Information: EGF-containing fibulin-like extracellular matrix protein 1
• UniProt Protein Name: EGF-containing fibulin-like extracellular matrix protein 1
• Protein Family: EGF-containing fibulin-like extracellular matrix protein
• UniProt Gene Name: EFEMP1
• UniProt Synonym Gene Names: FBLN3; FBNL; FIBL-3
• UniProt Entry Name: FBLN3_HUMAN

NCBI Description

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; epidermal growth factor receptor activity; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; visual perception; negative regulation of chondrocyte differentiation

Disease: Doyne Honeycomb Retinal Dystrophy