anti-Mouse CD28 Monoclonal Antibody | anti-CD28 antibody

Anti-Ms CD28 Purified Low Endotoxin

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by protein-G affinity chromatography
• Synonyms: CD28; Monoclonal Antibody; Anti-Ms CD28 Purified Low Endotoxin; Tp44; CD28 molecule; anti-CD28 antibody

• Host: Golden Syrian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Golden Syrian Hamster IgG
• Clone Number: 37.51
• Specificity: The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.
• Purity/Purification: Purified by protein-G affinity chromatography
• Form/Format: Purified Low Endotoxin. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Functional Application (FUNC)
• Application Notes: FUNC: In vitro and in vivo T cell costimulation.; FC/FACS: Recommended dilution: 6ug/ml; Immunocytochemistry: Recommended dilution: 1-4ug/ml IP: Recommended dilution: 1-2ug/100-500 ug of protein in 1 ml lysate.
• Immunogen: Mouse T cell lymphoma EL-4 cells
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of murine peritoneal fluid cells using anti-CD28 (clone 37.51) purified, GAArH-FITC.)

Related Product Information for anti-CD28 antibody

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 Functional Application (FUNC)tions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

References

*Zhang et al.: The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway. J Immunol. 2008 Apr 15;180(8):5506-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/18390734ordin,*Rosenberger CM, Clark AE, Treuting PM, Johnson CD, Aderem A: ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2544-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18268321ordin,*Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS: The related adaptors, adaptor in lymphocytes of unknown Functional Application (FUNC)tion X and Rlk/Itk-binding protein, have nonredundant Functional Application (FUNC)tions in lymphocytes. J Immunol. 2007 Aug 1;179(3):1768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/17641043ordin,*Pua HH, Dzhagalov I, Chuck M, Mizushima N, He YW: A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J Exp Med. 2007 Jan 22;204(1):25-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/17190837ordin,*Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS: Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006 Aug;26(16):6005-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/16880512ordin,*Zhang N, He YW: The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005 Jun 1;174(11):6967-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15905539ordin,*Zhang N, He YW: An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med. 2005 Aug 1;202(3):395-404., URL: https://www.ncbi.nlm.nih.gov/pubmed/16043517ordin,*Albert MH, Yu XZ, Martin PJ, Anasetti C: Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin. Blood. 2005 Feb 1;105(3):1355-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/15459004ordin,*Gross JA, Callas E, Allison JP: Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992 Jul 15;149(2):380-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1320641ordina,*Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP: CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature. 1992 Apr 16;356(6370):607-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1313950ordina

NCBI and Uniprot Product Information

• NCBI GI #: 161484597
• NCBI GeneID: 12487
• NCBI Accession #: NP_031668.3
• NCBI GenBank Nucleotide #: NM_007642.4
• UniProt Accession #: P31041; Q6GSH7
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.