Western Blot (WB)
(Western Blot (WB) analysis of IGHA1/IGHA2 pAb at 1:1000 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug))
Rabbit anti-Human, Mouse IgA Polyclonal Antibody | anti-IgA antibody
IgA (Y276) Polyclonal Antibody
Gene Names
IGHA1; IgA1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
IgA; Polyclonal Antibody; IgA (Y276) Polyclonal Antibody; Ig alpha-1 chain C region; IGHA1; Ig alpha-2 chain C region; IGHA2; anti-IgA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IgA (Y276) polyclonal antibody detects endogenous levels of IgA protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
353
Applicable Applications for anti-IgA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 241-290 of Human IgA.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of IGHA1/IGHA2 pAb at 1:1000 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of IGHA1/IGHA2 pAb at 1:1000 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of IgA (Y276) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of IgA (Y276) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-IgA antibody
Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~ 38kDa
NCBI Official Full Name
Immunoglobulin heavy constant alpha 1
NCBI Official Synonym Full Names
immunoglobulin heavy constant alpha 1
NCBI Official Symbol
IGHA1
NCBI Official Synonym Symbols
IgA1
UniProt Protein Name
Ig alpha-1 chain C region
Protein Family
UniProt Gene Name
IGHA1
UniProt Entry Name
IGHA1_HUMAN
Uniprot Description
IGHA1: Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. A chromosomal aberration involving IGHA1 is found in multiple myeloma (MM) cell lines. Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein.
Protein type: Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: extracellular space; extracellular region
Molecular Function: antigen binding
Biological Process: retinal homeostasis; receptor-mediated endocytosis; glomerular filtration; antibacterial humoral response; immune response; protein-chromophore linkage
Research Articles on IgA
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Product Notes
The IgA igha1 (Catalog #AAA3003880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IgA (Y276) Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IgA igha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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