Rabbit anti-Human, Mouse IFT88 Polyclonal Antibody | anti-IFT88 antibody

IFT88, CT (IFT88, TG737, TTC10, Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10) (APC)

Cat. Num. AAA6309410

• Gene Names: IFT88; DAF19; TG737; TTC10; hTg737; D13S1056E
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IFT88; Polyclonal Antibody; CT (IFT88; TG737; TTC10; Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10) (APC); Tetratricopeptide repeat protein 10; anti-IFT88 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-IFT88 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IFT88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 798-827 amino acids from the C-terminal region of human IFT88.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(IFT88 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IFT88 antibody detected the IFT88 protein (arrow).)

Western Blot (WB)

(IFT88 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the IFT88 antibody detected the IFT88 protein (arrow).)

Immunohistochemistry (IHC)

(IFT88 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IFT88 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of IFT88 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-IFT88 antibody

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].

Product Categories/Family for anti-IFT88 antibody

Antibodies; Tetratricopeptide Repeat Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Robert, A., et al. J. Cell. Sci. 120 (PT 4), 628-637 (2007): Khanna, H., et al. J. Biol. Chem. 280(39):33580-33587(2005). Lehner, B., et al. Genomics 83(1):153-167(2004). Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 28329439
• NCBI GeneID: 8100
• NCBI Accession #: NP_006522.2
• NCBI GenBank Nucleotide #: NM_006531.3
• UniProt Accession #: Q13099; Q5SZJ6; Q8N719; A2A491; B4DUS2
• Molecular Weight: 94,270 Da
• NCBI Official Full Name: intraflagellar transport protein 88 homolog isoform 2
• NCBI Official Synonym Full Names: intraflagellar transport 88
• NCBI Official Symbol: IFT88
• NCBI Official Synonym Symbols: DAF19; TG737; TTC10; hTg737; D13S1056E
• NCBI Protein Information: intraflagellar transport protein 88 homolog; polaris homolog; TPR repeat protein 10; tetratricopeptide repeat domain 10; intraflagellar transport 88 homolog; tetratricopeptide repeat protein 10; recessive polycystic kidney disease protein Tg737 homolog; p
• UniProt Protein Name: Intraflagellar transport protein 88 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT88
• UniProt Synonym Gene Names: TG737; TTC10; TPR repeat protein 10

Uniprot Description

Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.