Goat IFT74 / CCDC2 Polyclonal Antibody | anti-IFT74 antibody

Goat Polyclonal to Human IFT74 / CCDC2

Cat. Num. AAA241962

• Gene Names: IFT74; CMG1; CCDC2; CMG-1
• Reactivity: Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IFT74 / CCDC2; Polyclonal Antibody; Goat Polyclonal to Human IFT74 / CCDC2; Anti-IFT74 / CCDC2 Antibody (C-Terminus) IHC-plus; IFT74; CCDC2; CMG-1; CMG1; Human IFT74; anti-IFT74 antibody

• Host: Goat
• Reactivity: Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human IFT74.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 600

• Applicable Applications for anti-IFT74 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KTIVDALHSTSGN from the C-terminus of human IFT74 (NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Gibbon (92%); Rabbit (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: IFT74 / CCDC2 antibody was raised against synthetic peptide C-KTIVDALHSTSGN from the C-terminus of human IFT74 (NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Gibbon (92%); Rabbit (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-IFT74 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody staining (0.3 ug/ml) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 151105229
• NCBI GeneID: 80173
• NCBI Accession #: NP_079379.2
• NCBI GenBank Nucleotide #: NM_025103.2
• UniProt Accession #: Q96LB3; Q3B789; Q5VY34; Q6PGQ8; Q9H643; Q9H8G7
• Molecular Weight: 42,332 Da
• NCBI Official Full Name: intraflagellar transport protein 74 homolog isoform a
• NCBI Official Synonym Full Names: intraflagellar transport 74
• NCBI Official Symbol: IFT74
• NCBI Official Synonym Symbols: CMG1; CCDC2; CMG-1
• NCBI Protein Information: intraflagellar transport protein 74 homolog; coiled-coil domain containing 2; capillary morphogenesis protein 1; intraflagellar transport 74 homolog; capillary morphogenesis gene 1 protein; coiled-coil domain-containing protein 2
• UniProt Protein Name: Intraflagellar transport protein 74 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT74
• UniProt Synonym Gene Names: CCDC2; CMG1; CMG-1
• UniProt Entry Name: IFT74_HUMAN

Uniprot Description

IFT74: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9p21.2

Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; nucleus; cilium

Molecular Function: beta-tubulin binding; chromatin binding

Biological Process: Notch signaling pathway; organelle organization and biogenesis; cilium biogenesis; positive regulation of cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; negative regulation of epithelial cell proliferation