Western Blot (WB)
(Western blot (WB) analysis of IFN-? polyclonal antibody at 1:500 dilution
Lane1:The testis tissue lysate of Mouse(20ug)
Lane2:The spleen tissue lysate of Rat(20ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:U-87MG whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)
Lane6:BV2 whole cell lysate(40ug)
Lane7:PC3 whole cell lysate(40ug))
Rabbit IFN-alpha Polyclonal Antibody | anti-IFN-alpha antibody
IFN-alpha polyclonal antibody
Gene Names
IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Synonyms
IFN-alpha; Polyclonal Antibody; IFN-alpha polyclonal antibody; Aldehyde oxidase; Aldehyde oxidase 1; Azaheterocycle hydroxylase; AOX1; anti-IFN-alpha antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
IFN-alpha polyclonal antibody detects endogenous levels of IFN-alpha protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IFN-alpha antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500~1:1000
IF: 1:50~1:200
IF: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human IFN-alpha.
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of IFN-? polyclonal antibody at 1:500 dilution Lane1:The testis tissue lysate of Mouse(20ug) Lane2:The spleen tissue lysate of Rat(20ug) Lane3:A549 whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug) Lane6:BV2 whole cell lysate(40ug) Lane7:PC3 whole cell lysate(40ug))
Western Blot (WB)
(Western blot (WB) analysis of IFN-? polyclonal antibody at 1:500 dilution
Lane1:The testis tissue lysate of Mouse(20ug)
Lane2:The spleen tissue lysate of Rat(20ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:U-87MG whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)
Lane6:BV2 whole cell lysate(40ug)
Lane7:PC3 whole cell lysate(40ug))
Related Product Information for anti-IFN-alpha antibody
Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: alpha, beta and gamma. IFN-alpha is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-alpha from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-alpha induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway. IFN-alpha signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon). All IFN-alpha signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,725 Da
NCBI Official Full Name
Interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 1
NCBI Official Symbol
IFNA1
NCBI Official Synonym Symbols
IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
NCBI Protein Information
interferon alpha-1/13; leIF D; IFN-alpha 1b; IFN-alpha-1/13; interferon-alpha1; interferon alpha-D; interferon alpha 1b
UniProt Protein Name
Interferon alpha-1/13
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
NCBI Description
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
Research Articles on IFN-alpha
Similar Products
Product Notes
The IFN-alpha ifna1 (Catalog #AAA3005488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFN-alpha polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFN-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500~1:1000 IF: 1:50~1:200. Researchers should empirically determine the suitability of the IFN-alpha ifna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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