Rabbit anti-Human IFM2 Polyclonal Antibody | anti-IFM2 antibody

IFM2, NT (IFITM2, Interferon-induced transmembrane protein 2, Dispanin subfamily A member 2c, Interferon-inducible protein 1-8D) (MaxLight 550)

Cat. Num. AAA6309306

• Gene Names: IFITM2; 1-8D; DSPA2c
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IFM2; Polyclonal Antibody; NT (IFITM2; Interferon-induced transmembrane protein 2; Dispanin subfamily A member 2c; Interferon-inducible protein 1-8D) (MaxLight 550); IFITM2; Interferon-inducible protein 1-8D; anti-IFM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IFM2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-IFM2 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human IFM2, UniProt Accession #Q01629.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6005333. IFM2 (arrow) was detected usingMBS6005333.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma using MBS6005333 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005333 for immunohistochemistry.)

Product Categories/Family for anti-IFM2 antibody

Antibodies; Transmembrane Proteins

References

1. Daniel-Carmi, V., et al. Int. J. Cancer 125(12):2810-2819(2009). 2. Lehner, B., et al. Genome Res. 14(7):1315-1323(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 151101191
• NCBI GeneID: 10581
• NCBI Accession #: NP_006426.2
• NCBI GenBank Nucleotide #: NM_006435.2
• UniProt Accession #: Q01629; Q6FH82; Q96DA8
• Molecular Weight: 14,632 Da
• NCBI Official Full Name: interferon-induced transmembrane protein 2
• NCBI Official Synonym Full Names: interferon induced transmembrane protein 2
• NCBI Official Symbol: IFITM2
• NCBI Official Synonym Symbols: 1-8D; DSPA2c
• NCBI Protein Information: interferon-induced transmembrane protein 2
• UniProt Protein Name: Interferon-induced transmembrane protein 2
• UniProt Gene Name: IFITM2
• UniProt Synonym Gene Names: DSPA2c
• UniProt Entry Name: IFM2_HUMAN

Uniprot Description

IFITM2: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein- mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. Belongs to the CD225/Dispanin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: integral to membrane; plasma membrane

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; immune response; negative regulation of viral genome replication; negative regulation of virion penetration into host cell; response to virus