Goat IDH3G Polyclonal Antibody | anti-IDH3G antibody

Goat anti-IDH3G (aa337-350) Antibody

Cat. Num. AAA422664

• Gene Names: IDH3G; H-IDHG
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: IDH3G; Polyclonal Antibody; Goat anti-IDH3G (aa337-350) Antibody; isocitrate dehydrogenase 3 (NAD+) gamma; H-IDHG; IDH-gamma; NAD (H)-specific isocitrate dehydrogenase gamma subunit; NAD(+)-specific ICDH subunit gamma; NAD+-specific ICDH; OTTHUMP00000025984; OTTHUMP00000025987; OTTHUMP00000025988; isocitrate dehy; IDH3G antibody; isocitrate dehydrogenase 3 (NAD+) gamma antibody; H-IDHG antibody; IDH-gamma antibody; NAD (H)-specific isocitrate dehydrogenase gamma subunit antibody; NAD(+)-specific ICDH subunit gamma antibody; NAD+-specific ICDH antibody; OTTHUMP00000025984 antibody; OTTHUMP00000025987 antibody; OTTHUMP00000025988 antibody; isocitrate dehydrogenase [NAD] subunit gamma; mitochondrial antibody; isocitrate dehydrogenase; NAD(+)-specific; mitochondrial; gamma subunit antibody; isocitric dehydrogenase subunit gamma antibody; IDH3G (aa337-350); anti-IDH3G antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DHLKLHSYATSIRK
• Sequence Length: 393

• Applicable Applications for anti-IDH3G antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 38kDa band observed in lysates of cell line MCF7 and of Human, Mouse, Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2ug/ml.
• Immunogen: Peptide with sequence C-DHLKLHSYATSIRK, from the internal region of the protein sequence according to NP_004126.1; NP_777358.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Rat (A) and Pig (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.5ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758582
• NCBI GeneID: 3421
• NCBI Accession #: NP_004126.1; NP_777358.1
• NCBI GenBank Nucleotide #: NM_004135.3
• Molecular Weight: 41,468 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD(+)) gamma
• NCBI Official Symbol: IDH3G
• NCBI Official Synonym Symbols: H-IDHG
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3G
• UniProt Entry Name: IDH3G_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

IDH3G: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 may have tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: mitochondrial matrix; mitochondrion; nucleolus; nucleoplasm

Molecular Function: ATP binding; isocitrate dehydrogenase (NAD+) activity; magnesium ion binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; carbohydrate metabolic process; isocitrate metabolic process; NADH metabolic process; negative regulation of growth; tricarboxylic acid cycle