Rabbit IDH3G Polyclonal Antibody | anti-IDH3G antibody

IDH3G Rabbit pAb

Cat. Num. AAA4760002

• Gene Names: IDH3G; H-IDHG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: IDH3G; Polyclonal Antibody; IDH3G Rabbit pAb; isocitrate dehydrogenase 3 (NAD+) gamma; H-IDHG; anti-IDH3G antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.2mg/ml (varies by lot)

• Applicable Applications for anti-IDH3G antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; IHC-P: 1:25-1:100
• Immunogen: Fusion protein of human IDH3G
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of IDH3G in A431 cells and mouse brain tissue in order using IDH3G Rabbit pAb at dilution 1/300)

Immunohistochemistry (IHC)

(Immunohistochemistry of IDH3G in paraffin-embedded Human thyroid cancer tissue using IDH3G Rabbit pAb at dilution 1/30. Predicted cell location: Cytoplasm)

Related Product Information for anti-IDH3G antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.

NCBI and Uniprot Product Information

• NCBI GI #: 4758582
• NCBI GeneID: 3421
• NCBI Accession #: NP_004126.1
• NCBI GenBank Nucleotide #: NM_004135.3
• UniProt Accession #: P51553; Q9BUU5; E9PDD5
• Molecular Weight: 41,468 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD+) gamma
• NCBI Official Symbol: IDH3G
• NCBI Official Synonym Symbols: H-IDHG
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; IDH-gamma; NAD (H)-specific isocitrate dehydrogenase gamma subunit; NAD(+)-specific ICDH subunit gamma; NAD+-specific ICDH; isocitrat
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3G
• UniProt Entry Name: IDH3G_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

IDH3G: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 may have tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41; Mitochondrial

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; nucleolus

Molecular Function: magnesium ion binding; isocitrate dehydrogenase (NAD+) activity; NAD binding; ATP binding

Biological Process: isocitrate metabolic process; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process