Rabbit IDH2 Polyclonal Antibody | anti-IDH2 antibody

IDH2 Polyclonal Antibody

Cat. Num. AAA3011992

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IDH2; Polyclonal Antibody; IDH2 Polyclonal Antibody; Isocitrate dehydrogenase [NADP]; mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; anti-IDH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IDH2 polyclonal antibody detects endogenous levels of IDH2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 452

• Applicable Applications for anti-IDH2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:50; IP: 1:50-1:200; ChIP: 1:20-1:100
• Immunogen: Recombinant full length Human IDH2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using IDH2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using IDH2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-IDH2 antibody

IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.

NCBI and Uniprot Product Information

• NCBI GI #: 28178832
• NCBI GeneID: 3418
• NCBI Accession #: NP_002159.2
• NCBI GenBank Nucleotide #: NP_002159.2
• UniProt Accession #: P48735
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase (NADP(+)) 2
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]