Rabbit IDH1 Polyclonal Antibody | anti-IDH1 antibody

IDH1 Rabbit Polyclonal

Cat. Num. AAA7600350

• Gene Names: IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunoprecipitation, Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: IDH1; Polyclonal Antibody; IDH1 Rabbit Polyclonal; anti-IDH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 414

• Applicable Applications for anti-IDH1 antibody: ELISA (EIA), Immunoprecipitation (IP), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC)
• Immunogen: Isocitrate dehydrogenase 1 (NADP+), soluble
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gliomas tissue slide using MBS7600350 (IDH1 Antibody) at dilution of 1:50)

Western Blot (WB)

(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7600350 (IDH1 antibody) at dilution of 1:2000)

NCBI and Uniprot Product Information

• NCBI GI #: 538917681
• NCBI GeneID: 3417
• NCBI Accession #: NP_001269316.1
• NCBI GenBank Nucleotide #: NM_001282387.1
• Molecular Weight: 46,659 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• NCBI Official Symbol: IDH1
• NCBI Official Synonym Symbols: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH1
• UniProt Synonym Gene Names: PICD; IDH
• UniProt Entry Name: IDHC_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.1.1.42

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: cytoplasm; cytosol; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; NAD binding; NADP binding; protein homodimerization activity; receptor binding

Biological Process: 2-oxoglutarate metabolic process; female gonad development; glutathione metabolic process; glyoxylate cycle; isocitrate metabolic process; NADPH regeneration; response to oxidative stress; response to steroid hormone stimulus; tricarboxylic acid cycle

Disease: Glioma Susceptibility 1