Rabbit anti-Human, Mouse HES7 Polyclonal Antibody | anti-HES7 antibody

HES7, NT (HES7, BHLHB37, Transcription factor HES-7, Class B basic helix-loop-helix protein 37, Hairy and enhancer of split 7, bHLH factor Hes7) (FITC)

Cat. Num. AAA6306233

• Gene Names: HES7; SCDO4; bHLHb37
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HES7; Polyclonal Antibody; NT (HES7; BHLHB37; Transcription factor HES-7; Class B basic helix-loop-helix protein 37; Hairy and enhancer of split 7; bHLH factor Hes7) (FITC); bHLH factor Hes7; anti-HES7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HES7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HES7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~63 amino acids from the N-terminal region of human HES7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HES7 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). HES7 (arrow) was detected using the purified Pab.)

Related Product Information for anti-HES7 antibody

HES7 is a member of the hairy and enhancer of split family of bHLH transcription factors. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton.

Product Categories/Family for anti-HES7 antibody

Antibodies; Transcription Factors

References

Sparrow, D.B., et al. Hum. Mol. Genet. 17(23):3761-3766(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 260166650
• NCBI GeneID: 84667
• NCBI Accession #: NP_001159439.1
• NCBI GenBank Nucleotide #: NM_001165967.1
• UniProt Accession #: Q9BYE0; F8VPC9
• Molecular Weight: 25,280 Da
• NCBI Official Full Name: transcription factor HES-7 isoform 1
• NCBI Official Synonym Full Names: hes family bHLH transcription factor 7
• NCBI Official Symbol: HES7
• NCBI Official Synonym Symbols: SCDO4; bHLHb37
• NCBI Protein Information: transcription factor HES-7; bHLH factor Hes7; class B basic helix-loop-helix protein 37; hHes7; hairy and enhancer of split 7
• UniProt Protein Name: Transcription factor HES-7
• Protein Family: Transcription factor
• UniProt Gene Name: HES7
• UniProt Synonym Gene Names: BHLHB37; hHes7; bHLHb37
• UniProt Entry Name: HES7_HUMAN

NCBI Description

This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

HES7: Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation. Defects in HES7 are the cause of spondylocostal dysostosis type 4 (SCDO4). A rare condition of variable severity characterized by vertebral and costal anomalies. The main feature include dwarfism, vertebral fusion, hemivertebrae, posterior rib fusion, reduced rib number, and other rib malformations.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription factor binding

Biological Process: somitogenesis; Notch signaling pathway; transcription, DNA-dependent; rhythmic process; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; skeletal development

Disease: Spondylocostal Dysostosis 4, Autosomal Recessive