Rabbit DOK4 Polyclonal Antibody | anti-DOK4 antibody

DOK4 Antibody

Cat. Num. AAA9401282

• Gene Names: DOK4; IRS5; IRS-5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DOK4; Polyclonal Antibody; DOK4 Antibody; dockingprotein4; FLJ10488; Insulinreceptorsubstrate5; IRS5; anti-DOK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DOK4 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-DOK4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant Protein of human DOK4.
• Target Name: DOK4
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cells, using DOK4 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostate cancer using DOK4 Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using DOK4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney using DOK4 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using DOK4 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-DOK4 antibody

The downstream of kinase family (Dok-1-7) are members of a class of docking proteins that interact with receptor tyrosine kinases and, via this interaction, mediate biological responses within the body. Dok-4 (Downstream of kinase-4) is a 326 amino acid protein that contains one PH domain and one IRS-type PTB domain and belongs to the Dok family of interacting proteins. Expressed in a variety of tissues with highest expression in liver, heart, kidney and skeletal muscle, Dok-4 plays an important role in Ret-mediated neurite outgrowth and may link Ret with downstream effectors during neuronal differentiation. Additionally, Dok-4 is thought to play a positive role in the activation of MAPK pathways and may participate in T-cell induced immune system regulation. Overexpression of Dok-4 is associated with clear cell renal cell carcinoma, suggesting a role for Dok-4 in tumorigenesis.

Product Categories/Family for anti-DOK4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 20070302
• NCBI GeneID: 55715
• NCBI Accession #: NP_060580.2
• NCBI GenBank Nucleotide #: NM_018110.3
• UniProt Accession #: Q8TEW6; O75209; Q9BTP2; Q9NVV3
• Molecular Weight: 37,028 Da
• NCBI Official Full Name: docking protein 4
• NCBI Official Synonym Full Names: docking protein 4
• NCBI Official Symbol: DOK4
• NCBI Official Synonym Symbols: IRS5; IRS-5
• NCBI Protein Information: docking protein 4
• UniProt Protein Name: Docking protein 4
• Protein Family: Docking protein
• UniProt Gene Name: DOK4
• UniProt Synonym Gene Names: IRS-5; IRS5
• UniProt Entry Name: DOK4_HUMAN

Uniprot Description

DOK4: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. Interacts with Ret and TIE2. Interaction with Ret is mediated through the PTB domain and requires phosphorylation of Ret Y1062.

Protein type: Cell development/differentiation; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q21

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: nervous system development; MAPKKK cascade; transmembrane receptor protein tyrosine kinase signaling pathway