Rabbit anti-Human ICMT Polyclonal Antibody | anti-ICMT antibody

ICMT, CT (Protein-S-isoprenylcysteine O-methyltransferase, Isoprenylcysteine Carboxylmethyltransferase, Prenylcysteine Carboxyl Methyltransferase, pcCMT, Prenylated Protein Carboxyl Methyltransferase, PPMT, ICMT, PCCMT) (Biotin)

Cat. Num. AAA6483658

• Gene Names: ICMT; PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ICMT; Polyclonal Antibody; CT (Protein-S-isoprenylcysteine O-methyltransferase; Isoprenylcysteine Carboxylmethyltransferase; Prenylcysteine Carboxyl Methyltransferase; pcCMT; Prenylated Protein Carboxyl Methyltransferase; PPMT; PCCMT) (Biotin); anti-ICMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ICMT.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ICMT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ICMT (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-ICMT Pab is used in Western blot to detect ICMT in T47D (left) and SK-BR-3 (right) tissue lysate)

Product Categories/Family for anti-ICMT antibody

Antibodies; Enzymes; Methyltransferase

References

1. Bergo, M.O., et al., J. Clin. Invest. 113(4):539-550 (2004). 2. Ahmad, M., et al., Arterioscler. Thromb. Vasc. Biol. 22(5):759-764 (2002). 3. Van Dessel, G.A., et al., Int. J. Biochem. Cell Biol. 34(5):477-486 (2002). 4. Dai, Q., et al., J. Biol. Chem. 273(24):15030-15034 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 6912430
• NCBI GeneID: 23463
• NCBI Accession #: NP_036537
• NCBI GenBank Nucleotide #: NM_012405
• UniProt Accession #: O60725
• Molecular Weight: 32kDa
• NCBI Official Full Name: protein-S-isoprenylcysteine O-methyltransferase
• NCBI Official Synonym Full Names: isoprenylcysteine carboxyl methyltransferase
• NCBI Official Symbol: ICMT
• NCBI Official Synonym Symbols: PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098
• NCBI Protein Information: protein-S-isoprenylcysteine O-methyltransferase
• UniProt Protein Name: Protein-S-isoprenylcysteine O-methyltransferase
• Protein Family: Protein-S-isoprenylcysteine O-methyltransferase
• UniProt Gene Name: ICMT
• UniProt Synonym Gene Names: PCCMT; PPMT; pcCMT

NCBI Description

This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ICMT: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Belongs to the isoprenylcysteine O-methyltransferase family.

Protein type: EC 2.1.1.100; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Methyltransferase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: protein C-terminal carboxyl O-methyltransferase activity; protein-S-isoprenylcysteine O-methyltransferase activity

Biological Process: C-terminal protein methylation; cellular protein modification process; post-translational protein modification; protein targeting to membrane