Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IBTK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit anti-Human IBTK Polyclonal Antibody | anti-IBTK antibody
IBTK Polyclonal Antibody
Gene Names
IBTK; BTKI; BTBD26
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purification
Synonyms
IBTK; Polyclonal Antibody; IBTK Polyclonal Antibody; BTBD26; BTKI; anti-IBTK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
GLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQSPSSTGLLKSGKTNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTTGFHSDKIEAKVKPYVNGTSPVYSREDLKPWEKSPILKISAPQPIPSNRIDTTSSASWVAGSFSPVSPPVVDLRTIMEIEESRQKCGATPKSHLGKTVSHGVKLS
Sequence Length
1338
Applicable Applications for anti-IBTK antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human IBTK
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein
Positive Samples
Jurkat, HepG2
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IBTK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IBTK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-IBTK antibody
Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Categories/Family for anti-IBTK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 26kDa; 133kDa; 150kDa
Observed: 167kDa
Observed: 167kDa
NCBI Official Full Name
inhibitor of Bruton tyrosine kinase isoform 2
NCBI Official Synonym Full Names
inhibitor of Bruton tyrosine kinase
NCBI Official Symbol
IBTK
NCBI Official Synonym Symbols
BTKI; BTBD26
NCBI Protein Information
inhibitor of Bruton tyrosine kinase
UniProt Protein Name
Inhibitor of Bruton tyrosine kinase
Protein Family
UniProt Gene Name
IBTK
UniProt Synonym Gene Names
BTKI; KIAA1417; IBtk
NCBI Description
Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
Uniprot Description
Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.
Research Articles on IBTK
Similar Products
Product Notes
The IBTK ibtk (Catalog #AAA9134858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IBTK Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IBTK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the IBTK ibtk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GLNMAALLEA RSLDVLSDGV LKDLSEFYRK MIPAMDRRVI TPYQDGPDIS YLEVEDGDIF LKEEINMEQN HSETMFKKAK TKAKKKPRKR SDSSGGYNLS DIIQSPSSTG LLKSGKTNSV ESLPELLTSD SEGSYAGVGS PRDLQSPDFT TGFHSDKIEA KVKPYVNGTS PVYSREDLKP WEKSPILKIS APQPIPSNRI DTTSSASWVA GSFSPVSPPV VDLRTIMEIE ESRQKCGATP KSHLGKTVSH GVKLS. It is sometimes possible for the material contained within the vial of "IBTK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
