Rabbit anti-Human IBTK Polyclonal Antibody | anti-IBTK antibody

IBTK, ID (IBTK, BTKI, KIAA1417, Inhibitor of Bruton tyrosine kinase) (PE)

Cat. Num. AAA6309056

• Gene Names: IBTK; BTKI; BTBD26; RP1-93K22.1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IBTK; Polyclonal Antibody; ID (IBTK; BTKI; KIAA1417; Inhibitor of Bruton tyrosine kinase) (PE); Inhibitor of Bruton tyrosine kinase; anti-IBTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IBTK antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IBTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 589-617 amino acids from the Central region of human IBTK.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(IBTK Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IBTK antibody detected the IBTK protein (arrow).)

Immunohistochemistry (IHC)

(IBTK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IBTK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IBTK Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IBTK antibody

The protein encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription.

Product Categories/Family for anti-IBTK antibody

Antibodies; Protein Kinases

References

Fiume, G., et al. Comput Biol Chem 33(6):434-439(2009). Spatuzza, C., et al. Nucleic Acids Res. 36(13):4402-4416(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 57863304
• NCBI GeneID: 25998
• NCBI Accession #: NP_056340.2
• NCBI GenBank Nucleotide #: NM_015525.2
• UniProt Accession #: Q9P2D0; Q2QKU2; Q2QKU3; Q2QKU4; Q5TFD7; Q5TFD9; Q8IUQ9; Q8IUY7; Q8TAI4; Q9HBI8; Q9Y3T8
• Molecular Weight: 150,528 Da
• NCBI Official Full Name: inhibitor of Bruton tyrosine kinase
• NCBI Official Synonym Full Names: inhibitor of Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: IBTK
• NCBI Official Synonym Symbols: BTKI; BTBD26; RP1-93K22.1
• NCBI Protein Information: inhibitor of Bruton tyrosine kinase; BTK-binding protein; inhibitor of Bruton's tyrosine kinase; inhibitor of Bruton's tyrosine kinase-beta; inhibitor of Bruton's tyrosine kinase-alpha; inhibitor of Bruton's tyrosine kinase-gamma; Bruton agammaglobulinemi
• UniProt Protein Name: Inhibitor of Bruton tyrosine kinase
• Protein Family: Inhibitor of Bruton tyrosine kinase
• UniProt Gene Name: IBTK
• UniProt Synonym Gene Names: BTKI; KIAA1417; IBtk
• UniProt Entry Name: IBTK_HUMAN

NCBI Description

The protein encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription. Ref.7

Subunit structure: Interacts with the PH domain of BTK. Isoform 2 does not interact with BTK. Ref.1 Ref.7

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein. Note: Translocates to the plasma membrane upon IgM stimulation. Ref.1 Ref.7Isoform 2: Nucleus Ref.1 Ref.7.

Tissue specificity: Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues. Ref.1 Ref.7

Sequence similarities: Contains 3 ANK repeats.Contains 2 BTB (POZ) domains.Contains 3 RCC1 repeats.

Sequence caution: The sequence AAG27170.1 differs from that shown. Reason: Aberrant splicing.The sequence BAA92655.2 differs from that shown. Reason: Erroneous initiation. The sequence CAI23414.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23416.1 differs from that shown. Reason: Erroneous gene model prediction.