Rabbit iASPP Polyclonal Antibody | anti-iASPP antibody

RABBIT ANTI HUMAN iASPP

Cat. Num. AAA222822

• Gene Names: PPP1R13L; RAI; RAI4; IASPP; NKIP1
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: iASPP; Polyclonal Antibody; RABBIT ANTI HUMAN iASPP; anti-iASPP antibody

• Host: Rabbit
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 828

• Applicable Applications for anti-iASPP antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a bands of approximately 100 and 50kDa corresponding to iASPP isoforms 1 and 2 respectively.; ELISA: Minimum Dilution: 1/40000; Maximum Dilution: 1/160000; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human iASPP were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-iASPP antibody

Rabbit anti Human iASPP antibody detects human inhibitor of apoptosis stimulating protein of p53 (iASPP), which plays a central role in regulation of apoptosis and transcription via its interaction with NFkB and p53 proteins. At present, the iASPP gene is thought to encode three isoforms; isoform 1 is predominantly located in the cytoplasm, whilst isoforms 2 and 3 are nuclear proteins. iASPP is highly expressed in the heart, placenta and prostate. iASPP has been shown to have oncogenic properties in human breast carcinomas.

NCBI and Uniprot Product Information

• NCBI GI #: 215820635
• NCBI GeneID: 10848
• NCBI Accession #: NP_001135974.1
• NCBI GenBank Nucleotide #: NM_001142502.1
• UniProt Accession #: Q8WUF5; Q2PNZ9; Q5DU71; Q5I1X4; Q6P1R7; Q6PKF8; Q9Y290
• Molecular Weight: 89,091 Da
• NCBI Official Full Name: relA-associated inhibitor
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 13 like
• NCBI Official Symbol: PPP1R13L
• NCBI Official Synonym Symbols: RAI; RAI4; IASPP; NKIP1
• NCBI Protein Information: relA-associated inhibitor; NFkB interacting protein 1; NFkB-interacting protein 1; PPP1R13B-like protein; inhibitor of ASPP protein; inhibitor of apoptosis stimulating protein of p53; protein iASPP; protein phosphatase 1, regulatory (inhibitor) subunit 13 like; retinoic acid induced 4
• UniProt Protein Name: RelA-associated inhibitor
• UniProt Gene Name: PPP1R13L
• UniProt Synonym Gene Names: IASPP; NKIP1; PPP1R13BL; RAI; Protein iASPP
• UniProt Entry Name: IASPP_HUMAN

NCBI Description

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM, Mar 2008]

Uniprot Description

PPP1R13L: an oncoprotein that interacts with and inhibits NF-kappaB, Sp1 and p53. Plays a central role in regulation of apoptosis and transcription. May inhibit p53 by preventing its association with ASPP1 or ASPP2, thereby suppressing the subsequent activation of apoptosis. Required for neuronal survival after axonal injury. Two alternatively spliced isoforms have been described. Isoform 1 is preferrentially found in the cytoplasm; isoform 2 is nuclear.

Protein type: Apoptosis; Phosphatase, regulatory subunit; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: intercellular bridge; cytoplasm; nucleus; cell junction

Molecular Function: identical protein binding; protein binding; transcription factor binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; multicellular organismal homeostasis; embryonic camera-type eye development; post-embryonic development; cardiac muscle contraction; hair cycle