Western Blot (WB)
(Western blot analysis of HYOU1 antibody (Center) in 293 cell line lysates (35ug/lane). HYOU1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Hypoxia Up-regulated Protein 1 Polyclonal Antibody | anti-HYOU1 antibody
Hypoxia Up-regulated Protein 1 (HYOU1, 150kD Oxygen-regulated Protein, ORP150, ORP-150, 170kD Glucose-regulated Protein, Grp170, GRP-170, DKFZp686N08236, FLJ94899, FLJ97572, HSP12A) (PE)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of HYOU1 antibody (Center) in 293 cell line lysates (35ug/lane). HYOU1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HYOU1 antibody (Center) in 293 cell line lysates (35ug/lane). HYOU1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HYOU1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HYOU1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.
Protein type: Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 11q23.1-q23.3
Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region
Molecular Function: chaperone binding; ATP binding
Biological Process: receptor-mediated endocytosis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response
Research Articles on HYOU1
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Product Notes
The HYOU1 hyou1 (Catalog #AAA6481337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hypoxia Up-regulated Protein 1 (HYOU1, 150kD Oxygen-regulated Protein, ORP150, ORP-150, 170kD Glucose-regulated Protein, Grp170, GRP-170, DKFZp686N08236, FLJ94899, FLJ97572, HSP12A) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hypoxia Up-regulated Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HYOU1 hyou1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypoxia Up-regulated Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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