Rabbit anti-Human Hypoxia Up-regulated Protein 1 Polyclonal Antibody | anti-HYOU1 antibody

Hypoxia Up-regulated Protein 1 (HYOU1, 150kD Oxygen-regulated Protein, ORP150, ORP-150, 170kD Glucose-regulated Protein, Grp170, GRP-170, DKFZp686N08236, FLJ94899, FLJ97572, HSP12A) (APC)

Cat. Num. AAA6481328

• Gene Names: HYOU1; IMD59; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Hypoxia Up-regulated Protein 1; Polyclonal Antibody; Hypoxia Up-regulated Protein 1 (HYOU1; 150kD Oxygen-regulated Protein; ORP150; ORP-150; 170kD Glucose-regulated Protein; Grp170; GRP-170; DKFZp686N08236; FLJ94899; FLJ97572; HSP12A) (APC); anti-HYOU1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Hypoxia Up-regulated Protein 1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HYOU1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide between corresponding to a portion of amino acids within aa281-311 selected from the Center region of human HYOU1 conjugated to KLH.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HYOU1 antibody (Center) in 293 cell line lysates (35ug/lane). HYOU1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HYOU1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-HYOU1 antibody

Antibodies; Cancer Markers

References

1. Kitao, Y., Matsuyama, T. Antioxid. Redox Signal. 9(5), 589-595 (2007). 2. Bando, Y., Ogawa, S. Am. J. Physiol., Cell Physiol. 278(6), C1172-C1182 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 5453832
• NCBI GeneID: 10525
• NCBI Accession #: NP_006380
• NCBI GenBank Nucleotide #: NM_006389
• UniProt Accession #: Q9Y4L1
• Molecular Weight: 108kDa
• NCBI Official Full Name: hypoxia up-regulated protein 1
• NCBI Official Synonym Full Names: hypoxia up-regulated 1
• NCBI Official Symbol: HYOU1
• NCBI Official Synonym Symbols: IMD59; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• NCBI Protein Information: hypoxia up-regulated protein 1
• UniProt Protein Name: Hypoxia up-regulated protein 1
• Protein Family: Hypoxia up-regulated protein
• UniProt Gene Name: HYOU1
• UniProt Synonym Gene Names: GRP170; ORP150; ORP-150; GRP-170
• UniProt Entry Name: HYOU1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q23.1-q23.3

Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region

Molecular Function: chaperone binding; ATP binding

Biological Process: receptor-mediated endocytosis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response