Mouse CD25 Monoclonal Antibody | anti-CD25 antibody

MOUSE ANTI RAT CD25

Cat. Num. AAA212960

• Gene Names: Il2ra; IL2RAC
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD25; Monoclonal Antibody; MOUSE ANTI RAT CD25; anti-CD25 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: OX-39
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 267

• Applicable Applications for anti-CD25 antibody: Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50; Immunohistology - Paraffin: Application Note: PLP fixarion is recommended, see Whiteland. J.L. et al.(1995)for details.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin (MBS210477))

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)

Testing Data

(Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC (MBS216315))

Testing Data

(Published clone specific image: The percentage of Ly49s3+ NK cells increases in the spleen and decreases in the bone marrow following L. monocytogenes infection. A) Mononuclear leukocytes were harvested from spleen, blood, and bone marrow from uninfected and infected animals 24 and 48 hrs p.i. Cells were stained with antibodies to NKR-P1A, CD3, and Ly49s3. NK cells were gated as NKR-P1A+CD3- cells, and the percentage of Ly49s3+ NK cells was analysed by flow cytometry. The data represent the mean +/- SEM from three independent experiments, with 2 animals at each time point. Data were analyzed with the one-way ANOVA with Tukey's multiple comparisons test. B) The activation status of NK cells from uninfected (filled histogram) or infected animals 24 (grey line) and 48 hrs p.i. (black line) was tested by flow cytometry. An antibody towards the activation marker CD25 was employed.From: Shegarfi H, Naper C, Rolstad B, Inngjerdingen M (2010) Listeria monocytogenes Infection Affects a Subset of Ly49-Expressing NK Cells in the Rat. PLoS ONE 5(12): e15579.)

Testing Data

(Staining of rat spleen cells with Mouse anti Rat CD25: FITC (MBS210996))

Testing Data

(Published customer image: Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)

NCBI and Uniprot Product Information

• NCBI GI #: 7710038
• NCBI GeneID: 25704
• NCBI Accession #: NP_037295.1
• NCBI GenBank Nucleotide #: NM_013163.1
• Molecular Weight: 30,557 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: IL2RAC
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL-2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha chain
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_RAT

NCBI Description

alpha chain of the interleukin 2 receptor; human homolog homodimerizes to form low affinity receptor [RGD, Feb 2006]

Uniprot Description

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.