Rabbit anti-Human, Mouse Hypoxia Inducible Factor 3a Polyclonal Antibody | anti-Hif3a antibody

Hypoxia Inducible Factor 3a (Hypoxia Inducible Factor 3 alpha, Hypoxia Inducible Factor 3 alpha Subunit, Hypoxia Inducible Factor Three alpha, HIF-3a, HIF3A, HIF3 alpha, HIF-3A4, Inhibitory PAS Domain Protein, IPAS, MOP7, PASD7)

Cat. Num. AAA620016

• Gene Names: Hif3a; Mop7
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Purified; Purified
• Synonyms: Hypoxia Inducible Factor 3a; Polyclonal Antibody; Hypoxia Inducible Factor 3a (Hypoxia Inducible Factor 3 alpha; Hypoxia Inducible Factor 3 alpha Subunit; Hypoxia Inducible Factor Three alpha; HIF-3a; HIF3A; HIF3 alpha; HIF-3A4; Inhibitory PAS Domain Protein; IPAS; MOP7; PASD7); Anti -Hypoxia Inducible Factor 3a (Hypoxia Inducible Factor 3 alpha; anti-Hif3a antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This antibody is specific for HIF-3 alpha. Species Crossreactivity: Recognizes human and mouse HIF-3 alpha.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-Hif3a antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
• Immunogen: A synthetic peptide made to an internal region of the human HIF-3 alpha (within residues 250-350). [Swiss-Prot# Q66K72].
• Positive Control: Cos7 CoCl2-treated lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Hif3a antibody

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Product Categories/Family for anti-Hif3a antibody

Antibodies; Abs to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 11968112
• NCBI GeneID: 64345
• NCBI Accession #: NP_071973.1
• NCBI GenBank Nucleotide #: NM_022528.1
• UniProt Accession #: Q9JHS2
• Molecular Weight: 72,888 Da
• NCBI Official Full Name: hypoxia-inducible factor 3-alpha
• NCBI Official Synonym Full Names: hypoxia inducible factor 3, alpha subunit
• NCBI Official Symbol: Hif3a
• NCBI Official Synonym Symbols: Mop7
• NCBI Protein Information: hypoxia-inducible factor 3-alpha; HIF3-alpha; HIF-3 alpha; HIF-3-alpha; HIF3 alpha 1; HIF3-alpha-1; hypoxia inducible factor 3a; hypoxia inducible factor 3 alpha; hypoxia-inducible factor 3 alpha
• UniProt Protein Name: Hypoxia-inducible factor 3-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif3a
• UniProt Entry Name: HIF3A_RAT

NCBI Description

binds HIF-responsive elements and activates transcription in vitro [RGD]

Uniprot Description

HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Transcription factor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; signal transduction