Goat HYPE/FICD Polyclonal Antibody | anti-FICD antibody
Goat anti-HYPE/FICD Antibody
Gene Names
FICD; HYPE; HIP13; UNQ3041
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HYPE/FICD; Polyclonal Antibody; Goat anti-HYPE/FICD Antibody; FICD; FIC domain containing; HIP13; HYPE; MGC5623; UNQ3041; Huntingtin interacting protein E; fic S-phase protein cell division homolog; huntingtin interacting protein 13; huntingtin interactor protein E; FICD antibody; FIC domain containing antibody; HIP13 antibody; HYPE antibody; MGC5623 antibody; UNQ3041 antibody; Huntingtin interacting protein E antibody; fic S-phase protein cell division homolog antibody; huntingtin interacting protein 13 antibody; HYPE / FICD; anti-FICD antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PPITIRKEQRSD
Sequence Length
458
Applicable Applications for anti-FICD antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of Mouse and Rat Liver and of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51.8kDa according to NP_009007.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of Mouse and Rat Liver and of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51.8kDa according to NP_009007.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-PPITIRKEQRSD, from the internal region of the protein sequence according to NP_009007.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,778 Da
NCBI Official Full Name
adenosine monophosphate-protein transferase FICD
NCBI Official Synonym Full Names
FIC domain containing
NCBI Official Symbol
FICD
NCBI Official Synonym Symbols
HYPE; HIP13; UNQ3041
NCBI Protein Information
adenosine monophosphate-protein transferase FICD
UniProt Protein Name
Adenosine monophosphate-protein transferase FICD
Protein Family
UniProt Gene Name
FICD
UniProt Synonym Gene Names
HIP13; HYPE; HIP-13
UniProt Entry Name
FICD_HUMAN
Uniprot Description
FICD: Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified. Belongs to the fic family.
Protein type: EC 2.7.7.n1; Adenylyltransferase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q24.1
Cellular Component: integral to membrane
Molecular Function: ATP binding
Biological Process: protein amino acid adenylylation
Research Articles on FICD
Similar Products
Product Notes
The FICD ficd (Catalog #AAA422690) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HYPE/FICD Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HYPE/FICD can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of Mouse and Rat Liver and of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51.8kDa according to NP_009007.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the FICD ficd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPITIRKEQR SD. It is sometimes possible for the material contained within the vial of "HYPE/FICD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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