CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric) Assay Kit
CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)
Synonyms
CytoSelect 48-well Cell Adhesion Assay (Laminin; Fluorometric); Fluorometric) assay kit
Preparation and Storage
Store all kit components at 4 degree C up to their expiration dates.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric) assay kit
Principle of the Assay: The CytoSelect Cell Adhesion Assay Kit utilizes a Laminin-coated 48-well plate (see Adhesion Plate Layout). First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are removed with consecutive washes. Finally, the adherent cells are lysed and subsequently detected with CyQuant GR Dye.
Background: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Mouse Laminin I-coated wells, 8 BSA-coated wells).
Background: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Mouse Laminin I-coated wells, 8 BSA-coated wells).
Product Categories/Family for CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric) assay kit
References
1. Hynes, R. O. (1992) Cell 69, 11-25.
2. Schwartz, M. A., Schaller, M. D. and Ginsberg, M. H. (1995) Annu. Rev. Cell Dev. Biol. 11, 549-599.
Lee, J. et al. (2013). Selective inhibition of prostaglandin E2 receptors EP2 and EP4 inhibits adhesion of human endometriotic epithelial and stromal cells through suppression of integrin-mediated mechanisms. Biol Reprod. 88:77.
2. Schwartz, M. A., Schaller, M. D. and Ginsberg, M. H. (1995) Annu. Rev. Cell Dev. Biol. 11, 549-599.
Lee, J. et al. (2013). Selective inhibition of prostaglandin E2 receptors EP2 and EP4 inhibits adhesion of human endometriotic epithelial and stromal cells through suppression of integrin-mediated mechanisms. Biol Reprod. 88:77.
