Rabbit anti-Human HYOU1 Polyclonal Antibody | anti-HYOU1 antibody

HYOU1 Polyclonal Antibody

Cat. Num. AAA9125443

• Gene Names: HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HYOU1; Polyclonal Antibody; HYOU1 Polyclonal Antibody; Grp170; HSP12A; ORP150; GRP-170; ORP-150; anti-HYOU1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 999

• Applicable Applications for anti-HYOU1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human HYOU1
• Calculated Molecular Weight: 111kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HYOU1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15min.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human vermiform appendix using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse testis using HYOU1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse spinal cord using HYOU1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-HYOU1 antibody

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-HYOU1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 10720185
• NCBI GeneID: 10525
• UniProt Accession #: Q9Y4L1; Q2I204; Q53H25; A8C1Z0; B7Z909
• Molecular Weight: 999
• NCBI Official Full Name: Hypoxia up-regulated protein 1
• NCBI Official Synonym Full Names: hypoxia up-regulated 1
• NCBI Official Symbol: HYOU1
• NCBI Official Synonym Symbols: Grp170; HSP12A; ORP150; GRP-170; ORP-150
• NCBI Protein Information: hypoxia up-regulated protein 1; 150 kDa oxygen-regulated protein; oxygen regulated protein (150kD); 170 kDa glucose-regulated protein
• UniProt Protein Name: Hypoxia up-regulated protein 1
• Protein Family: Hypoxia up-regulated protein
• UniProt Gene Name: HYOU1
• UniProt Synonym Gene Names: GRP170; ORP150; ORP-150; GRP-170
• UniProt Entry Name: HYOU1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 11q23.1-q23.3

Cellular Component: focal adhesion; smooth endoplasmic reticulum; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region

Molecular Function: chaperone binding; ATP binding

Biological Process: receptor-mediated endocytosis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response