Mouse anti-Human HYOU1 Polyclonal Antibody | anti-HYOU1 antibody

HYOU1 (GRP170, ORP150, Hypoxia Up-regulated Protein 1, 150kD Oxygen-regulated Protein, 170kD Glucose-regulated Protein)

Cat. Num. AAA6005177

• Gene Names: HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HYOU1; Polyclonal Antibody; HYOU1 (GRP170; ORP150; Hypoxia Up-regulated Protein 1; 150kD Oxygen-regulated Protein; 170kD Glucose-regulated Protein); Anti -HYOU1 (GRP170; anti-HYOU1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HYOU1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPVEDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSVCRASVTRFGF

• Applicable Applications for anti-HYOU1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full-length human HYOU1, aa1-678 (AAH72436.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HYOU1 polyclonal antibody. Western Blot analysis of HYOU1 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of HYOU1 expression in transfected 293T cell line by HYOU1 polyclonal antibody. Lane 1: HYOU1 transfected lysate (74.58kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-HYOU1 antibody

HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3aa shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.

Product Categories/Family for anti-HYOU1 antibody

Antibodies; Abs to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 47938913
• NCBI GeneID: 10525
• UniProt Accession #: Q9Y4L1; Q2I204; Q53H25; A8C1Z0
• Molecular Weight: 111,335 Da
• NCBI Official Full Name: HYOU1 protein
• NCBI Official Synonym Full Names: hypoxia up-regulated 1
• NCBI Official Symbol: HYOU1
• NCBI Official Synonym Symbols: Grp170; HSP12A; ORP150; GRP-170; ORP-150
• NCBI Protein Information: hypoxia up-regulated protein 1; glucose-regulated protein 170; 150 kDa oxygen-regulated protein; oxygen regulated protein (150kD); 170 kDa glucose-regulated protein
• UniProt Protein Name: Hypoxia up-regulated protein 1
• Protein Family: Hypoxia up-regulated protein
• UniProt Gene Name: HYOU1
• UniProt Synonym Gene Names: GRP170; ORP150; ORP-150; GRP-170
• UniProt Entry Name: HYOU1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq, Oct 2008]

Uniprot Description

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 11q23.1-q23.3

Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region

Molecular Function: chaperone binding; ATP binding

Biological Process: receptor-mediated endocytosis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response