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Western Blot (WB) (Western blotAll lanes: HYAL3 antibody at 4ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 47,44,19,16 kDaObserved band size: 46 kDa)

Rabbit anti-Human, Mouse HYAL3 Polyclonal Antibody | anti-HYAL3 antibody

HYAL3 Antibody

Gene Names
HYAL3; LUCA3; HYAL-3; LUCA-3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
HYAL3; Polyclonal Antibody; HYAL3 Antibody; Hyal-3; Hyaluronoglucosaminidase-3; Lung carcinoma protein 3; LuCa-3; LUCA3; anti-HYAL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
417
Applicable Applications for anti-HYAL3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:200-1000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Hyaluronidase-3 protein (198-417aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49211
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: HYAL3 antibody at 4ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 47,44,19,16 kDaObserved band size: 46 kDa)

Western Blot (WB) (Western blotAll lanes: HYAL3 antibody at 4ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 47,44,19,16 kDaObserved band size: 46 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1495314 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1495314 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS1495314 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine using MBS1495314 at dilution 1:100)
Related Product Information for anti-HYAL3 antibody
This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,865 Da
NCBI Official Full Name
hyaluronidase-3 isoform 1
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 3
NCBI Official Symbol
HYAL3
NCBI Official Synonym Symbols
LUCA3; HYAL-3; LUCA-3
NCBI Protein Information
hyaluronidase-3
UniProt Protein Name
Hyaluronidase-3
UniProt Gene Name
HYAL3
UniProt Synonym Gene Names
LUCA3; Hyal-3; LuCa-3
UniProt Entry Name
HYAL3_HUMAN

NCBI Description

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

Uniprot Description

HYAL3: a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

Protein type: Secreted; Secreted, signal peptide; Hydrolase; EC 3.2.1.35

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasmic vesicle; extracellular region; lysosome

Molecular Function: hyaluronoglucuronidase activity; hyalurononglucosaminidase activity; protein binding; viral receptor activity

Biological Process: carbohydrate metabolic process; cartilage development; hyaluronan catabolic process; inflammatory response; response to antibiotic; response to virus

Research Articles on HYAL3

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Product Notes

The HYAL3 hyal3 (Catalog #AAA1495314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HYAL3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HYAL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:200-1000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the HYAL3 hyal3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HYAL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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