Chicken Forkhead Box Protein P3 ELISA Kit | FOXP3 elisa kit

Chicken Forkhead Box Protein P3 ELISA Kit

Cat. Num. AAA736438

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Chicken
• Synonyms: Forkhead Box Protein P3; Chicken Forkhead Box Protein P3 ELISA Kit; FOXP3 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FOXP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken FOXP3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXP3 elisa kit

Principle of the assay: FOXP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOXP3 antibody and an FOXP3-HRP conjugate. The assay sample and buffer are incubated together with FOXP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOXP3 concentration since FOXP3 from samples and FOXP3-HRP conjugate compete for the anti-FOXP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOXP3 from the sample, fewer sites are left to bind FOXP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOXP3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FOXP3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0
• Molecular Weight: 47,244 Da
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Cell cycle regulation; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; NF-kappaB binding; protein binding; NFAT protein binding; protein homodimerization activity; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription factor activity; transcription corepressor activity

Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; myeloid cell homeostasis; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; response to virus; negative regulation of transcription factor activity; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of interferon-gamma biosynthetic process; negative regulation of histone acetylation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; regulation of isotype switching to IgG isotypes; positive regulation of T cell anergy; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked