Immunofluorescence (IF)
(Immunofluorescent ICC Analysis: HeLa cells were fixed, permeabilized, and stained using Anti-HuR (Cy3, Red). Cells were co-stained for actin (Phalloidin, AlexaFluor 488 conjugate, Green) and DAPI (nuclei, blue).)
Rabbit HuR Polyclonal Antibody | anti-HuR antibody
HuR (Hu antigen R, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1, ELAV1, ELAV-like protein 1, Hua, Hu-antigen R, HUR, MelG) (FITC)
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Immunofluorescent ICC Analysis: HeLa cells were fixed, permeabilized, and stained using Anti-HuR (Cy3, Red). Cells were co-stained for actin (Phalloidin, AlexaFluor 488 conjugate, Green) and DAPI (nuclei, blue).)
Immunofluorescence (IF)
(Immunofluorescent ICC Analysis: HeLa cells were fixed, permeabilized, and stained using Anti-HuR (Cy3, Red). Cells were co-stained for actin (Phalloidin, AlexaFluor 488 conjugate, Green) and DAPI (nuclei, blue).)
Immunocytochemistry (ICC)
(Immunofluorescent ICC Analysis: NIH/3T3, A431, and HeLa cells (Top, Middle, and Bottom, respectively) were grown, fixed, and permeabilized. The cells were stained using Anti-HuR (Cy3, Red), Phalloidin (actin fillaments, green), and DAPI (nuclei, blue). Images on the left are without DAPI filter and the images on the right show the DAPI filter. HuR staining overlays the nuclear staining.)
Immunocytochemistry (ICC)
(Immunofluorescent ICC Analysis: NIH/3T3, A431, and HeLa cells (Top, Middle, and Bottom, respectively) were grown, fixed, and permeabilized. The cells were stained using Anti-HuR (Cy3, Red), Phalloidin (actin fillaments, green), and DAPI (nuclei, blue). Images on the left are without DAPI filter and the images on the right show the DAPI filter. HuR staining overlays the nuclear staining.)
Western Blot (WB)
(Western Blot Analysis: Jurkat cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-HuR (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates HuR (36kD).)
Western Blot (WB)
(Western Blot Analysis: Jurkat cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with anti-HuR (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates HuR (36kD).)
NCBI and Uniprot Product Information
Uniprot Description
HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus
Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding
Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression
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Product Notes
The HuR elavl1 (Catalog #AAA6481255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HuR (Hu antigen R, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1, ELAV1, ELAV-like protein 1, Hua, Hu-antigen R, HUR, MelG) (FITC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HuR can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). WB: 1:500-1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HuR elavl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HuR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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