Rabbit Humanin Polyclonal Antibody | anti-MT-RNR2 antibody

Rabbit Polyclonal to Humanin

Cat. Num. AAA243560

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Antiserum
• Synonyms: Humanin; Polyclonal Antibody; Rabbit Polyclonal to Humanin; Anti-Humanin Antibody (aa1-24) IHC-plus; anti-MT-RNR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 1-24 of human humanin protein were used as the immunogen.
• Purity/Purification: Antiserum
• Form/Format: 50% glycerol, 0.025% sodium azide.
• Sequence Length: 24

• Applicable Applications for anti-MT-RNR2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:100 - 1:1000), IHC-P (1:100), WB (1:2000); Optimal dilution to be determined by the researcher.
• Target: Humanin
• Immunogen Species: Humanin antibody was raised against Human
• Immunogen Type: Recombinant protein
• Epitope: aa1-24
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-HUMANIN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of Humanin in (A) recombinant fusion protein containing amino acids1-24 and (B) fusion partner without these amino acids, using antibody at 1:2000.)

NCBI and Uniprot Product Information

• NCBI GI #: 52783077
• UniProt Accession #: Q8IVG9
• NCBI Official Full Name: Humanin
• UniProt Protein Name: Humanin
• Protein Family: Humanin
• UniProt Gene Name: MT-RNR2
• UniProt Entry Name: HUNIN_HUMAN

Uniprot Description

HUNIN: Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP. Belongs to the humanin family.