Rabbit anti-Human NOD1 Polyclonal Antibody | anti-NOD1 antibody

Rabbit Polyclonal (IgG) to Human NOD1

Cat. Num. AAA244927

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Unpurified serum
• Synonyms: NOD1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human NOD1; Anti-NOD1 Antibody (aa930-949) IHC-plus; CARD4; CLR7.1; NLRC1; Human NOD1; anti-NOD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Amino acids 930-949 of human Nod1 protein were used as the immunogen.
• Purity/Purification: Unpurified serum
• Form/Format: 50% glycerol, 0.025% sodium azide.
• Sequence Length: 953

• Applicable Applications for anti-NOD1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:100), WB (1:1000 - 1:2500)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NOD1.
• Immunogen Type: Synthetic peptide
• Immunogen: NOD1 antibody was raised against synthetic peptide from human NOD1.
• Antigen Modification: aa930-949
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NOD1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using antibody at 1:2500.)

NCBI and Uniprot Product Information

• NCBI GI #: 5174617
• NCBI GeneID: 10392
• NCBI Accession #: NP_006083.1
• NCBI GenBank Nucleotide #: NM_006092.2
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; B4DTU3
• Molecular Weight: 27,949 Da
• NCBI Official Full Name: nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1; NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; caspase recruitment domain-containing protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4
• UniProt Entry Name: NOD1_HUMAN

NCBI Description

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: basolateral plasma membrane; apical plasma membrane; cytoplasm; cytosol

Molecular Function: identical protein binding; peptidoglycan binding; protein binding; protein homodimerization activity; CARD domain binding; caspase activator activity; ATP binding

Biological Process: detection of bacterium; apoptosis; activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; stress-activated MAPK cascade; positive regulation of JNK cascade; positive regulation of caspase activity; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; interleukin-8 biosynthetic process; JNK cascade; detection of biotic stimulus; inflammatory response; toll-like receptor 4 signaling pathway; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway