Rabbit anti-Human, Mouse HtrA1 Polyclonal Antibody | anti-HtrA1 antibody

HtrA1, NT (Serine Protease HTRA1, L56, Serine Protease 11, HTRA, PRSS11) (FITC)

Cat. Num. AAA6481209

• Gene Names: HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HtrA1; Polyclonal Antibody; NT (Serine Protease HTRA1; L56; Serine Protease 11; HTRA; PRSS11) (FITC); anti-HtrA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HtrA1 at ~51kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HtrA1 antibody: Western Blot (WB), FLISA, Immunohistochemistry (IHC)
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa116-147 in the N-terminal region of human HtrA1, UniProt Accession #NP_002766; Q92743
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-HtrA1 N-term Pab is used in Western blot to detect HtrA1in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(HtrA1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HtrA1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-HtrA1 antibody

Antibodies; Apoptosis

References

1. Howes, N., et al., Clin Gastroenterol Hepatol 2(3):252-261 (2004). 2. Chien, J., et al., Oncogene 23(8):1636-1644 (2004). 3. Hu, S.I., et al., J. Biol. Chem. 273(51):34406-34412 (1998). 4. Zumbrunn, J., et al., Genomics 45(2):461-462 (1997). 5. Zumbrunn, J., et al., FEBS Lett. 398 (2-3), 187-192 (1996). 6. Angela Chamberland, et al. J. Biol. Chem. 2009; 284:27352-27359.

NCBI and Uniprot Product Information

• NCBI GI #: 4506141
• NCBI GeneID: 5654
• NCBI Accession #: NP_002766
• NCBI GenBank Nucleotide #: NM_002775
• UniProt Accession #: Q92743
• Molecular Weight: 53kDa
• NCBI Official Full Name: serine protease HTRA1
• NCBI Official Synonym Full Names: HtrA serine peptidase 1
• NCBI Official Symbol: HTRA1
• NCBI Official Synonym Symbols: L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• NCBI Protein Information: serine protease HTRA1
• UniProt Protein Name: Serine protease HTRA1
• Protein Family: Serine protease
• UniProt Gene Name: HTRA1
• UniProt Synonym Gene Names: HTRA; PRSS11
• UniProt Entry Name: HTRA1_HUMAN

NCBI Description

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

Uniprot Description

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular matrix; extracellular space; cytosol

Molecular Function: insulin-like growth factor binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of defense response to virus; regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway

Disease: Macular Degeneration, Age-related, 7; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy