Rabbit HTATSF1 Polyclonal Antibody | anti-HTATSF1 antibody

HTATSF1 Antibody

Cat. Num. AAA9611828

• Gene Names: HTATSF1; TATSF1; TAT-SF1; dJ196E23.2
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Rabbit
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: HTATSF1; Polyclonal Antibody; HTATSF1 Antibody; Cofactor required for Tat activation of HIV 1 transcription; dJ196E23.2; HIV Tat specific factor 1; HTSF-1; HTSF1; TAT SF1; Tat-SF1; anti-HTATSF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HTATSF1 Antibody detects endogenous levels of HTATSF1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 755

• Applicable Applications for anti-HTATSF1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human HTATSF1.
• Tissue Specificity: Widely expressed.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using HTATSF1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-HTATSF1 antibody

Description: Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.

Subunit Structure: Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with GTF2F2/RAP30 and POLR2A. Interacts with TCERG1/CA150. Interacts with SF3A2/SAP62 and the spliceosomal U small nuclear ribonucleoproteins (snRNPs).

Similarity: The RRM domains mediate interaction with U snRNPs. Belongs to the HTATSF1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 253970456
• NCBI GeneID: 27336
• NCBI Accession #: NP_001156752.1
• NCBI GenBank Nucleotide #: NP_001156752.1
• UniProt Accession #: O43719
• Molecular Weight: Observed: 140 kDa; Predicted: 86 kDa
• NCBI Official Full Name: HIV Tat-specific factor 1
• NCBI Official Synonym Full Names: HIV-1 Tat specific factor 1
• NCBI Official Symbol: HTATSF1
• NCBI Official Synonym Symbols: TATSF1; TAT-SF1; dJ196E23.2
• NCBI Protein Information: HIV Tat-specific factor 1
• UniProt Protein Name: HIV Tat-specific factor 1
• Protein Family: HIV Tat-specific factor
• UniProt Gene Name: HTATSF1
• UniProt Synonym Gene Names: Tat-SF1
• UniProt Entry Name: HTSF1_HUMAN

NCBI Description

The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009]