Rabbit anti-Human EIF1AY Polyclonal Antibody | anti-EIF1AY antibody

EIF1AY, NT (EIF1AY, Eukaryotic translation initiation factor 1A, Y-chromosomal, Eukaryotic translation initiation factor 4C) (PE)

Cat. Num. AAA6294818

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF1AY; Polyclonal Antibody; NT (EIF1AY; Eukaryotic translation initiation factor 1A; Y-chromosomal; Eukaryotic translation initiation factor 4C) (PE); Eukaryotic translation initiation factor 4C; anti-EIF1AY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EIF1AY antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF1AY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF1AY.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF1AY Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EIF1AY antibody detected the EIF1AY protein (arrow).)

Immunohistochemistry (IHC)

(EIF1AY Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF1AY Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-EIF1AY antibody

This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

Product Categories/Family for anti-EIF1AY antibody

Antibodies; Translation Initiation Factors

References

Serajee, F.J., et al. J. Child Neurol. 24(10):1258-1261(2009). Lim, J., et al. Cell 125(4):801-814(2006). Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004): Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004). Skaletsky, H., et al. Nature 423(6942):825-837(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 33356163
• NCBI GeneID: 9086
• NCBI Accession #: NP_004672.2
• NCBI GenBank Nucleotide #: NM_004681.2
• UniProt Accession #: O14602; Q9BS77
• Molecular Weight: 16,442 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 1A, Y-chromosomal
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 1A, Y-linked
• NCBI Official Symbol: EIF1AY
• NCBI Protein Information: eukaryotic translation initiation factor 1A, Y-chromosomal; eIF-4C; eIF-1A Y isoform; eukaryotic translation initiation factor 4C; eukaryotic translation initiation factor 1A, Y chromosome
• UniProt Protein Name: Eukaryotic translation initiation factor 1A, Y-chromosomal
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF1AY
• UniProt Synonym Gene Names: eIF-1A Y isoform; eIF-4C
• UniProt Entry Name: IF1AY_HUMAN

NCBI Description

This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF1AY: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: Yq11.223

Molecular Function: protein binding; translation initiation factor activity

Biological Process: translational initiation