Rabbit anti-Human HSPD1 Polyclonal Antibody | anti-hspd1 antibody

HSPD1, ID (60kD Heat Shock Protein, Mitochondrial, Heat Shock Protein 60, 60kD Chaperonin, Chaperonin 60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, HuCHA60, HSP60)

Cat. Num. AAA627836

• Gene Names: hspd1; hld4; cpn60; groel; hsp60; hsp65; spg13; chaperonin
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: HSPD1; Polyclonal Antibody; ID (60kD Heat Shock Protein; Mitochondrial; Heat Shock Protein 60; 60kD Chaperonin; Chaperonin 60; Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; HuCHA60; HSP60); Anti -HSPD1; anti-hspd1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSPD1.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-hspd1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human HSPD1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HSPD1 Antibody (Center) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data .)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.)

Related Product Information for anti-hspd1 antibody

HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.

Product Categories/Family for anti-hspd1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47938737
• NCBI GeneID: 399217
• NCBI Official Full Name: Hspd1 protein
• NCBI Official Synonym Full Names: heat shock 60kDa protein 1 (chaperonin)
• NCBI Official Symbol: hspd1
• NCBI Official Synonym Symbols: hld4; cpn60; groel; hsp60; hsp65; spg13; chaperonin
• NCBI Protein Information: heat shock 60kDa protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin)
• Protein Family: 60 kDa heat shock protein