Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HSPC150/UBE2T Polyclonal Antibody | anti-UBE2T antibody
Goat anti-HSPC150/UBE2T Antibody
Western Blot: Approx.22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
Uniprot Description
UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: ATP binding; chromatin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: DNA repair; protein autoubiquitination; protein monoubiquitination; response to DNA damage stimulus
Disease: Fanconi Anemia, Complementation Group T
Research Articles on UBE2T
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Product Notes
The UBE2T ube2t (Catalog #AAA420613) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HSPC150/UBE2T Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPC150/UBE2T can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx.22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the UBE2T ube2t for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLVGIEKKFH PDV. It is sometimes possible for the material contained within the vial of "HSPC150/UBE2T, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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