Rabbit anti-Human HspBP1 Polyclonal Antibody | anti-HspBP1 antibody

HspBP1 (FL4) Antibody

Cat. Num. AAA440079

• Gene Names: HSPBAP1; PASS1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: HspBP1; Polyclonal Antibody; HspBP1 (FL4) Antibody; anti-HspBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human HspBP1
• Form/Format: 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.200 mg/ml (varies by lot)
• Sequence Length: 488

• Applicable Applications for anti-HspBP1 antibody: Western Blot (WB)
• Origin: HspBP1 (FL4) is provided as an affinity purified rabbit polyclonal antibody, raised against a truncation mutant that cotains amino acids 84-359 of human recombinant HspBP1 (3).
• Immunogen: a truncation mutant that cotains amino acids 84-359 of human recombinant HspBP1 (3).
• License: (FL4)HspBP1 is sold under License from Desert Genetics, Inc. under United States Patent 6,410,713
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB)

Related Product Information for anti-HspBP1 antibody

HspBP1 is an Hsp70-interacting protein that was first isolated from a human heart cDNA library using the yeast two-hybrid system (1). The ATPase domain of Hsp70 binds HspBP1 and upon binding inhibits Hsp70 chaperone activity. More recently HspBP1 was found to be a homologue of the yeast Fes1 and Sls1 proteins and promotes nucleotide dissociation from Hsc70. HspBP1 has also been shown to strip nucleotide from yeast Ssa1p, making it a member of the class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity (2). HspBP1 is highly expressed in heart, brain, skeletal muscle, and pancreas tissues. It is also expressed in pancreatic, hepatic, and astrocytic cell lines.

References

1.) Alberti S, Bohse K, Arndt V, Schmitz A, Hohfeld J. 2004. The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator. Mol Biol Cell. 2004 Sep;15(9):4003-10. 2.) Raynes DA, Thomson CA, Stroster J, Newton T, Cuneo P, Guerriero V. 2006. Human serum contains detectable levels of the Hsp70 cochaperone HspBP1 and antibodies bound to HspBP1. J Immunoassay Immunochem. 27(3):251-64.

1. Raynes DA, Guerriero V Jr. 1998. Inhibition of Hsp70 ATPase activity and protein renaturation by a novel Hsp70-binding protein. J. Biol Chem. 273(49):32883-32888.2. Kabani M, McLellan C, Raynes DA, Guerriero V, Brodsky JL. 2002. HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc-70 nucleotide exchange factor. FEBS Lett. 531(2):339-342.3. McLellan CA, Raynes DA, Guerriero V. 2003. HspBP1, an Hsp70 cochaperone, has two structural domains and is capable of altering the conformation of the Hsp70 ATPase domain. J. Biol. Chem. 278(21):19017-19022.

NCBI and Uniprot Product Information

• NCBI GI #: 21314714
• NCBI GeneID: 79663
• NCBI Accession #: NP_078886.2
• NCBI GenBank Nucleotide #: NM_024610.5
• UniProt Accession #: Q96EW2; Q6P476; Q8N8J4; Q8NHH6; Q8WWF0
• Molecular Weight: 32,266 Da
• NCBI Official Full Name: HSPB1-associated protein 1
• NCBI Official Synonym Full Names: HSPB (heat shock 27kDa) associated protein 1
• NCBI Official Symbol: HSPBAP1
• NCBI Official Synonym Symbols: PASS1
• NCBI Protein Information: HSPB1-associated protein 1; 27 kDa heat shock protein-associated protein 1; protein associating with small stress protein PASS1
• UniProt Protein Name: HSPB1-associated protein 1
• Protein Family: Hsp70-binding protein
• UniProt Gene Name: HSPBAP1
• UniProt Synonym Gene Names: PASS1
• UniProt Entry Name: HBAP1_HUMAN

NCBI Description

This gene encodes a protein that binds to one of the small heat shock proteins, specifically hsp27. Hsp27 is involved with cell growth and differentiation. This encoded protein was found to be abnormally expressed in patients with intractable epilepsy, although how brain function is affected remains unknown. [provided by RefSeq, Sep 2011]

Uniprot Description

HSPBAP1: May play a role in cellular stress response. A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma. Translocation t(2;3)(q35;q21) with the putative pseudogene DIRC3. Produces an hybrid mRNA encoding a truncated HSPBAP1 lacking the first 36 amino acids. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: cytoplasm