Rabbit anti-Human, Rat HSPA2 Polyclonal Antibody | anti-HSPA2 antibody

HSPA2 Antibody

Cat. Num. AAA1492068

• Gene Names: HSPA2; HSP70-2; HSP70-3
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: HSPA2; Polyclonal Antibody; HSPA2 Antibody; Heat shock 70 kDa protein 2; anti-HSPA2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 639

• Applicable Applications for anti-HSPA2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Recommended dilution: WB:1:500-5000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Heat shock-related 70 kDa protein 2 protein (400-639aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160010
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: HSPA2 antibody at 6ug/mlLane 1:A431 whole cell lysateLane 2:Hela whole cell lysateLane 3:Rat brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 70kDaObserved band size: 70kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis using MBS1492068 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating HSPA2 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1492068 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1492068(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))

Related Product Information for anti-HSPA2 antibody

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

NCBI and Uniprot Product Information

• NCBI GI #: 13676857
• NCBI GeneID: 3306
• NCBI Accession #: NP_068814.2
• NCBI GenBank Nucleotide #: NM_021979.3
• UniProt Accession #: P54652; Q15508; Q53XM3; Q9UE78
• Molecular Weight: 70,021 Da
• NCBI Official Full Name: heat shock-related 70 kDa protein 2
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 2
• NCBI Official Symbol: HSPA2
• NCBI Official Synonym Symbols: HSP70-2; HSP70-3
• NCBI Protein Information: heat shock-related 70 kDa protein 2
• UniProt Protein Name: Heat shock-related 70 kDa protein 2
• Protein Family: Heat shock-related 70 kDa protein
• UniProt Gene Name: HSPA2
• UniProt Synonym Gene Names: Heat shock 70 kDa protein 2
• UniProt Entry Name: HSP72_HUMAN

Uniprot Description

HSPA2: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Interacts with ZNF541. Component of the CatSper complex. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: cell surface; cytosol; male germ cell nucleus; membrane; mitochondrion; myelin sheath; nucleus; synaptonemal complex

Molecular Function: ATP binding; enzyme binding; glycolipid binding; protein binding; unfolded protein binding

Biological Process: G2/M-specific positive regulation of cyclin-dependent protein kinase activity; male meiosis; male meiosis I; protein refolding; response to cold; response to heat; response to unfolded protein; spermatid development; spermatogenesis