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Western Blot (WB) (Fig.1. Western Blot analysis of various cells using HSP70 Polyclonal Antibody diluted at 1:1000.)

Rabbit HSP70 Polyclonal Antibody | anti-HSP70 antibody

HSP70 Polyclonal Antibody

Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
HSP70; Polyclonal Antibody; HSP70 Polyclonal Antibody; Rabbit Anti-HSP70 Polyclonal Antibody; HSPA1L; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 1-Hom; HSP70-Hom; HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; anti-HSP70 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HSP70 Polyclonal Antibody detects endogenous levels of HSP70 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
641
Applicable Applications for anti-HSP70 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
ELISA: 1:40000
Immunogen
Synthesized peptide derived from the internal region of human HSP70 at AA range: 190-270
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of various cells using HSP70 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB) (Fig.1. Western Blot analysis of various cells using HSP70 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB)

(Fig.2. Western Blot analysis of MCF-7 cells using HSP70 Polyclonal Antibody diluted at 1:1000.)

Western Blot (WB) (Fig.2. Western Blot analysis of MCF-7 cells using HSP70 Polyclonal Antibody diluted at 1:1000.)
Related Product Information for anti-HSP70 antibody
HSPA1L encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, Heat shock 70 kDa protein 1-like stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
heat shock 70 kDa protein 1-like
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 1 like
NCBI Official Symbol
HSPA1L
NCBI Official Synonym Symbols
HSP70T; hum70t; HSP70-1L; HSP70-HOM
NCBI Protein Information
heat shock 70 kDa protein 1-like
UniProt Protein Name
Heat shock 70 kDa protein 1-like
Protein Family
UniProt Gene Name
HSPA1L
UniProt Synonym Gene Names
Heat shock 70 kDa protein 1L; HSP70-Hom
UniProt Entry Name
HS71L_HUMAN

NCBI Description

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008]

Uniprot Description

HSPA1L: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Not induced by heat shock. Expressed in spermatids. Belongs to the heat shock protein 70 family.

Protein type: Mitochondrial; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: signalosome; nucleoplasm; mitochondrial matrix; cytosol

Molecular Function: protein binding; unfolded protein binding; ATP binding

Biological Process: binding of sperm to zona pellucida; protein refolding; response to unfolded protein

Research Articles on HSP70

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Product Notes

The HSP70 hspa1l (Catalog #AAA9702277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP70 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 ELISA: 1:40000. Researchers should empirically determine the suitability of the HSP70 hspa1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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