Rabbit anti-Human UBE2C Polyclonal Antibody | anti-UBE2C antibody

UBE2C Polyclonal Antibody

Cat. Num. AAA3011189

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE2C; Polyclonal Antibody; UBE2C Polyclonal Antibody; Cyclin selective ubiquitin carrier protein; dJ447F3.2; Mitotic specific ubiquitin conjugating enzyme; UB E2C; UBCH 10; UbcH10; UBE 2C; Ube2c; UBE2C_HUMAN; Ubiquitin carrier protein C; Ubiquitin carrier protein E2 C; Ubiquitin carrier protein E2C; Ubiquitin conjugating enzyme E2 C; Ubiquitin conjugating enzyme E2C; Ubiquitin protein ligase C; Ubiquitin-conjugating enzyme E2 C; Ubiquitin-protein ligase C; anti-UBE2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE2C polyclonal antibody detects endogenous levels of UBE2C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 132

• Applicable Applications for anti-UBE2C antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:100; IP: 1:20-1:50
• Immunogen: Recombinant full length Human UBE2C.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of UBE2C polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells, using UBE2C polyclonal antibody)

Related Product Information for anti-UBE2C antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.

NCBI and Uniprot Product Information

• NCBI GI #: 528524529
• NCBI GeneID: 11065
• NCBI Accession #: NP_001268670.1
• NCBI GenBank Nucleotide #: NP_001268670.1
• UniProt Accession #: O00762
• Molecular Weight: ~19kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 6
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10
• UniProt Entry Name: UBE2C_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ligase; Cell cycle regulation; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; anaphase-promoting complex; cytoplasm; cytosol

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; protein ubiquitination; cyclin catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; exit from mitosis; positive regulation of exit from mitosis