Rabbit anti-Human HSP27 Polyclonal Antibody | anti-HSP27 antibody

HSP27 Rabbit Polyclonal

Cat. Num. AAA7600277

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: HSP27; Polyclonal Antibody; HSP27 Rabbit Polyclonal; anti-HSP27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 205

• Applicable Applications for anti-HSP27 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Heat shock 27kDa protein 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7600277 (HSP27 antibody) at dilution of 1:200)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7600277 (HSPB1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 11036357
• NCBI GeneID: 3315
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: HSP27
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Heat shock protein; Chaperone; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; nucleus; plasma membrane; proteasome complex; spindle; Z disc

Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: cell motility; negative regulation of apoptosis; negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of autophagy; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mRNA stability; regulation of translational initiation; response to unfolded protein; response to virus; retinal homeostasis; vascular endothelial growth factor receptor signaling pathway

Disease: Charcot-marie-tooth Disease, Axonal, Type 2f; Neuronopathy, Distal Hereditary Motor, Type Iib