HSP27 recombinant protein

Recombinant HSP27

Cat. Num. AAA201131

• Gene Names: Hspb1; 27kDa; Hsp25
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: HSP27; Recombinant HSP27; 27kDa; Hsp25; Heat shock 27kDa protein; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen regulated 24 kDa protein; Estrogen-regulated 24 kDa protein; Heat shock 25kDa protein 1; Heat shock 27 kDa protein; Heat shock 27kD protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat shock protein beta-1; heat shock protein family B (small) member 1; HEL-S-102; HMN2B; HS.76067; Hsp 25; HSP 27; Hsp 28; Hsp B1; Hsp28; HspB1; HSPB1_HUMAN; SRP27; Stress responsive protein 27; Stress-responsive protein 27; HSP27 recombinant protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl (pH8.0) Containing 10% Glycerol, 1mM DTT.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 1-209aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MGS MTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK
• Sequence Length: 209

• Applicable Applications for HSP27 recombinant protein: SDS-PAGE
• Species: Mouse
• Tag: His-Tag
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for HSP27 recombinant protein

HSP27, also known as heat shock protein beta-1, is one of the small heat shock proteins and constitutively expressed in various tissues. HSP27 expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor-BB(PDGF-BB)-stimulated migration of osteoblast-like MC3T3-E1 cells. Recombinant mouse HSP27, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for HSP27 recombinant protein

Signal Transduction

References

Kainuma S., et al. (2017) Int. J Mol. Med. 40(4):1057-66. Butt E., et al. (2001) J. Biol. Chem. 276(10): 7108-113.

NCBI and Uniprot Product Information

• NCBI GI #: 158937312
• NCBI GeneID: 15507
• NCBI Accession #: NP_038588.2
• NCBI GenBank Nucleotide #: NP_038588.2
• UniProt Accession #: P14602
• Molecular Weight: 25.4kDa (232aa) confirmed by MALDI-TOF
• NCBI Official Full Name: heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein 1
• NCBI Official Symbol: Hspb1
• NCBI Official Synonym Symbols: 27kDa; Hsp25
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: Hspb1
• UniProt Synonym Gene Names: Hsp25; Hsp27; HspB1; HSP 25; HSP 27

Uniprot Description

Involved in stress resistance and actin organization.