Human Basic Salivary Proline Rich Protein 2 ELISA Kit | PRB2 elisa kit

Human Basic Salivary Proline Rich Protein 2 ELISA Kit

Cat. Num. AAA764710

• Gene Names: PRB2; Ps; cP7; IB-9; PRPPRB1
• Reactivity: Human
• Synonyms: Basic Salivary Proline Rich Protein 2; Human Basic Salivary Proline Rich Protein 2 ELISA Kit; PRB2; PRB2 elisa kit

• Reactivity: Human
• Sequence Length: 416

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PRB2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PRB2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PRB2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PRB2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PRB2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 209571537
• NCBI GeneID: 653247
• NCBI Accession #: NP_006239.3
• NCBI GenBank Nucleotide #: NM_006248.3
• Molecular Weight: 40,799 Da
• NCBI Official Full Name: basic salivary proline-rich protein 2 preproprotein
• NCBI Official Synonym Full Names: proline-rich protein BstNI subfamily 2
• NCBI Official Symbol: PRB2
• NCBI Official Synonym Symbols: Ps; cP7; IB-9; PRPPRB1
• NCBI Protein Information: basic salivary proline-rich protein 2
• UniProt Protein Name: Basic salivary proline-rich protein 2
• Protein Family: Basic salivary proline-rich protein
• UniProt Gene Name: PRB2
• UniProt Synonym Gene Names: Salivary proline-rich protein
• UniProt Entry Name: PRB2_HUMAN

NCBI Description

This gene encodes a member of the heterogeneous family of basic, proline-rich, human salivary glycoproteins. The encoded preproprotein undergoes proteolytic processing to generate one or more mature isoforms before secretion from the parotid glands. Multiple alleles of this gene exhibiting variations in the length of the tandem repeats, polymorphic cleavage sites and polymorphic stop codons have been identified. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. [provided by RefSeq, Oct 2015]

Uniprot Description

PRB2: N- and O-glycosylated. In head and neck cancer patients, O-glycosylated with glucosylgalactosyl carbohydrate moiety. This modification would require prior hydroxylation on the lysine residue. Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P3 position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation occurs on terminal Gln residues of cleaved peptides. Pyroglutamate formation found on at least Gln-398 and Gln-400.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: extracellular region