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Western Blot (WB) (Western blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1, 000 dilution.)

Rabbit Cpn10 Monoclonal Antibody | anti-Cpn10 antibody

Cpn10 Antibody

Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Cpn10; Monoclonal Antibody; Cpn10 Antibody; 10 kDa chaperonin antibody; 10 kDa heat shock protein mitochondrial antibody; 10 kDa heat shock protein; mitochondrial antibody; CH10_HUMAN antibody; Chaperonin 10 antibody; Chaperonin 10 homolog antibody; CPN10 antibody; cpn10 homolog antibody; Early pregnancy factor antibody; Early-pregnancy factor antibody; EPF antibody; GROES antibody; GroES homolog antibody; Heat shock 10kD protein 1 chaperonin 10 antibody; Heat shock 10kDa protein 1 antibody; Heat shock 10kDa protein 1 chaperonin 10 antibody; Heat shock protein family E (Hsp10) member antibody; Heat-shock 10-kD protein antibody; Hsp10 antibody; Hspe1 antibody; anti-Cpn10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG82-34
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Cpn10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human Cpn10 aa 1-150.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1, 000 dilution.)

Western Blot (WB) (Western blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-Cpn10 antibody
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 10, a 102 amino acid protein, forms a heptameric ring of seven identical subunits. This ring binds at either end of HSP 60 to form a functional heterodimer.
Product Categories/Family for anti-Cpn10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,932 Da
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 10kDa protein 1 (chaperonin 10)
NCBI Official Symbol
HSPE1
NCBI Official Synonym Symbols
EPF; CPN10; GROES; HSP10
NCBI Protein Information
10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
UniProt Entry Name
CH10_HUMAN

NCBI Description

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Uniprot Description

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrion; mitochondrial matrix

Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein

Research Articles on Cpn10

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Product Notes

The Cpn10 hspe1 (Catalog #AAA9436672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cpn10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cpn10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:10-1:50. Researchers should empirically determine the suitability of the Cpn10 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cpn10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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