Western Blot (WB)
(Western Blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1,000 dilution.)
Rabbit Cpn10 Polyclonal Antibody | anti-Cpn10 antibody
Cpn10 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Cpn10; Polyclonal Antibody; Cpn10 Polyclonal Antibody; Hsp10; CPN10; EPF; HSPE1; 10 kDa chaperonin; 10 kDa heat shock protein mitochondrial; 10 kDa heat shock protein; mitochondrial; CH10; Chaperonin 10; Chaperonin 10 homolog; cpn10 homolog; Early pregnancy factor; Early-pregnancy factor; GROES; GroES homolog; Heat shock 10kD protein 1 chaperonin 10; Heat shock 10kDa protein 1; Heat shock 10kDa protein 1 chaperonin 10; Heat shock protein family E (Hsp10) member; Heat-shock 10-kD protein; Hspe1; anti-Cpn10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cpn10 polyclonal antibody detects endogenous levels of Cpn10 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
102
Applicable Applications for anti-Cpn10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2,000
IP: 1:10-1:50
IHC: 1:50-1:200
IP: 1:10-1:50
IHC: 1:50-1:200
Immunogen
Recombinant protein within human Cpn10 aa 1-150.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1,000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-Cpn10 antibody
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 10, a 102 amino acid protein, forms a heptameric ring of seven identical subunits. This ring binds at either end of HSP 60 to form a functional heterodimer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11kDa
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
UniProt Entry Name
CH10_HUMAN
Uniprot Description
HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: membrane; mitochondrion; mitochondrial matrix
Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding
Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein
Similar Products
Product Notes
The Cpn10 hspe1 (Catalog #AAA3012505) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cpn10 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cpn10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2,000 IP: 1:10-1:50 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Cpn10 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cpn10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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